Genomic Exploration of the Hemiascomycetous Yeasts:: 11.: Kluyveromyces lactis

Random sequencing of the Kluyveromyces lactis genome allowed the identification of 2235-2601 open reading frames (ORFs) homologous to S. cervisiae ORFs, 51 ORFs which were homologous to genes from other species, 64 tRNAs, the complete rDNA repeat, and a few Ty1- and Ty2-like sequences. In addition, the complete sequence of plasmid pKD1 and a large coverage of the mitochondrial genome were obtained. The global distribution into general functional categories found in Saccharomyces cervisiae and as defined by MIPS is well conserved in K. lactis, However, detailed examination of certain subcategories revealed a small excess of genes involved in amino acid metabolism in K, lactis, The sequences are deposited at Eh EMBL under the accession numbers,AL324881-AL430960. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.