Use of tapered amplifier diode laser for biological-friendly high-resolution optical trapping

被引：17

作者：

Cheng, Wei ^{[1
]}

Hou, Ximiao ^{[1
]}

Ye, Fangmao ^{[1
]}

机构：

[1] Univ Michigan, Coll Pharm, Dept Pharmaceut Sci, Ann Arbor, MI 48109 USA

来源：

OPTICS LETTERS | 2010年 / 35卷 / 17期

关键词：

RNA-POLYMERASE; TWEEZERS; TRAPS; EXCITATION; OXYGEN; DAMAGE;

D O I：

10.1364/OL.35.002988

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A 1064 nm laser is commonly used for biological optical trapping. However, it has the problem of generating reactive oxygen species in the presence of a sensitizer, which leads to photo damage in biological samples. Here we constructed optical tweezers using a tapered amplifier diode laser that operates at 830 nm. Compared to a 1064 nm laser, this laser is friendly to live cells, eliminates photo damage associated with reactive oxygen species, and allows simultaneous two-photon fluorescence imaging of green fluorescent proteins in live mammalian cells. All these advantages could significantly benefit future application of this single molecule technique in biological studies. (C) 2010 Optical Society of America

引用

页码：2988 / 2990

页数：3

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