Use of an immunomagnetic separation-fluorescent immunoassay (IMS-FIA) for rapid and high throughput analysis of environmental water samples

被引：26

作者：

Yu, H ^{[1
]}

机构：

[1] Veridian Corp, Calspan Operat, Buffalo, NY 14225 USA

来源：

ANALYTICA CHIMICA ACTA | 1998年 / 376卷 / 01期

关键词：

immunomagnetic separation; environmental water sample; bacteria and toxin detection; magnetic bead washer;

D O I：

10.1016/S0003-2670(98)00440-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An antibody-based immunomagnetic separation (IMS) in conjunction with microplate fluorescent immunoassay (FIA) was used for rapid screening of bacteria and toxin in environmental water samples. This approach allows processing and analysis of up to 96 samples on the same microplate within 1 h of assay time. The assay sensitivities are at the level of 2 x 10(3) cells for Escherichia coli and 10 ng/ml for Staphylococcal enterotoxin B detection, respectively. This unique IMS-FIA approach is also suitable for diner crude sample processing and analysis, as well as for system automation. (C) 1998 Published by Elsevier Science B.V. All rights reserved.

引用

页码：77 / 81

页数：5

共 12 条

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