Detection of bacteria by time-resolved laser-induced breakdown spectroscopy

被引:163
作者
Morel, S [1 ]
Leone, N
Adam, P
Amouroux, J
机构
[1] Ctr Etud Bouchet, Direct Gen Armement, F-91710 Vert Le Petit, France
[2] Ctr Etud Bouchet, Direct Ctr Expertises & Essais, F-91710 Vert Le Petit, France
[3] Ecole Natl Super Chim Paris, Lab Genie Procedes Plasmas & Traitements Surfaces, F-75231 Paris 05, France
关键词
D O I
10.1364/AO.42.006184
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
A laser-induced breakdown spectroscopy technique for analyzing biological matter for the detection of biological hazards is investigated. Eight species were considered in our experiment: six bacteria and two pollens in pellet form. The experimental setup is described, then a cumulative intensity ratio is proposed as a quantitative criterion because of its linearity and reproducibility. Time-resolved laser-induced breakdown spectroscopy (TRELIBS) exhibits a good ability to differentiate among all these species, whatever the culture medium, the species or the strain. Thus we expect that TRELIBS will be a good candidate for a sensor of hazards either on surfaces or in ambient air. (C) 2003 Optical Society of America.
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页码:6184 / 6191
页数:8
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