Single-Molecule Studies of RNA Polymerase: One Singular Sensation, Every Little Step It Takes

被引：70

作者：

Larson, Matthew H. ^{[2
]}

Landick, Robert ^{[4
]}

Block, Steven M. ^{[1
,3
]}

机构：

[1] Stanford Univ, Dept Appl Phys, Stanford, CA 94305 USA

[2] Stanford Univ, Biophys Program, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Biol, Stanford, CA 94305 USA

[4] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

来源：

MOLECULAR CELL | 2011年 / 41卷 / 03期

关键词：

INTRINSIC TRANSCRIPTION TERMINATION; NANO-POSITIONING SYSTEM; II ELONGATION COMPLEX; STRUCTURAL BASIS; NASCENT RNA; IN-VIVO; CHAIN ELONGATION; NONTEMPLATE DNA; HIGH-RESOLUTION; TRIGGER LOOP;

D O I：

10.1016/j.molcel.2011.01.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcription is the first of many biochemical steps that turn the genetic information found in DNA into the proteins responsible for driving cellular processes. In this review, we highlight certain advantages of single-molecule techniques in the study of prokaryotic and eukaryotic transcription, and the specific ways in which these techniques complement conventional, ensemble-based biochemistry. We focus on recent literature, highlighting examples where single-molecule methods have provided fresh insights into mechanism. We also present recent technological advances and outline future directions in the field.

引用

页码：249 / 262

页数：14

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