Quantitative metabolome analysis using capillary electrophoresis mass spectrometry

被引：751

作者：

Soga, T ^{[1
]}

Ohashi, Y

Ueno, Y

Naraoka, H

Tomita, M

Nishioka, T

机构：

[1] Keio Univ, Inst Adv Biosci, Yamagata 9970017, Japan

[2] Kyoto Univ, Grad Sch Agr Sci, Kyoto 6068502, Japan

来源：

JOURNAL OF PROTEOME RESEARCH | 2003年 / 2卷 / 05期

关键词：

metabolomics; metabolic pathways; capillary electrophoresis; mass spectrometry; Bacillus subtilis; sporulation; glycolysis; TCA; nucleotide; CoA;

D O I：

10.1021/pr034020m

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new approach for the comprehensive and quantitative analysis of charged metabolites by capillary electrophoresis mass spectrometry (CE-MS) is proposed. Metabolites are first separated by CE based on charge and size and then selectively detected using MS by monitoring over a large range of m/z values. This method enabled the determination of 352 metabolic standards and its utility was demonstrated in the analysis of 1692 metabolites from Bacillus subtilis extracts, revealing significant changes in metabolites during B. subtilis sporulation.

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收藏

页码：488 / 494

页数：7

相关论文

共 32 条

[1]

Aharoni Asaph, 2002, OMICS A Journal of Integrative Biology, V6, P217, DOI 10.1089/15362310260256882

[2] Quantitative characterization of differential ion suppression on liquid chromatography/atmospheric pressure ionization mass spectrometric bioanalytical methods [J].

Avery, MJ .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2003, 17 (03) :197-201

[3] Genome-wide analysis of the stationary-phase sigma factor (sigma-H) regulon of Bacillus subtilis [J].

Britton, RA ;

Eichenberger, P ;

Gonzalez-Pastor, JE ;

Fawcett, P ;

Monson, R ;

Losick, R ;

Grossman, AD .

JOURNAL OF BACTERIOLOGY, 2002, 184 (17) :4881-4890

[4] Analysis of peptides, proteins, protein digests, and whole human blood by capillary electrophoresis electrospray ionization mass spectrometry using an in-capillary electrode sheathless interface [J].

Cao, P ;

Moini, M .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1998, 9 (10) :1081-1088

[5] An optimized protocol for metabolome analysis in yeast using direct infusion electrospray mass spectrometry [J].

Castrillo, JI ;

Hayes, A ;

Mohammed, S ;

Gaskell, SJ ;

Oliver, SG .

PHYTOCHEMISTRY, 2003, 62 (06) :929-937

[6] Metabolic modeling of microbial strains in silico [J].

Covert, MW ;

Schilling, CH ;

Famili, I ;

Edwards, JS ;

Goryanin, II ;

Selkov, E ;

Palsson, BO .

TRENDS IN BIOCHEMICAL SCIENCES, 2001, 26 (03) :179-186

[7]

ERRINGTON J, 1993, MICROBIOL REV, V57, P1

[8] The transcriptional profile of early to middle sporulation in Bacillus subtilis [J].

Fawcett, P ;

Eichenberger, P ;

Losick, R ;

Youngman, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (14) :8063-8068

[9] Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry [J].

Fiehn, O ;

Kopka, J ;

Trethewey, RN ;

Willmitzer, L .

ANALYTICAL CHEMISTRY, 2000, 72 (15) :3573-3580

[10] Metabolite profiling for plant functional genomics [J].

Fiehn, O ;

Kopka, J ;

Dörmann, P ;

Altmann, T ;

Trethewey, RN ;

Willmitzer, L .

NATURE BIOTECHNOLOGY, 2000, 18 (11) :1157-1161

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