Quantitative metabolome analysis using capillary electrophoresis mass spectrometry

被引：751

作者：

Soga, T ^{[1
]}

Ohashi, Y

Ueno, Y

Naraoka, H

Tomita, M

Nishioka, T

机构：

[1] Keio Univ, Inst Adv Biosci, Yamagata 9970017, Japan

[2] Kyoto Univ, Grad Sch Agr Sci, Kyoto 6068502, Japan

来源：

JOURNAL OF PROTEOME RESEARCH | 2003年 / 2卷 / 05期

关键词：

metabolomics; metabolic pathways; capillary electrophoresis; mass spectrometry; Bacillus subtilis; sporulation; glycolysis; TCA; nucleotide; CoA;

D O I：

10.1021/pr034020m

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new approach for the comprehensive and quantitative analysis of charged metabolites by capillary electrophoresis mass spectrometry (CE-MS) is proposed. Metabolites are first separated by CE based on charge and size and then selectively detected using MS by monitoring over a large range of m/z values. This method enabled the determination of 352 metabolic standards and its utility was demonstrated in the analysis of 1692 metabolites from Bacillus subtilis extracts, revealing significant changes in metabolites during B. subtilis sporulation.

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收藏

页码：488 / 494

页数：7

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