Functional organization of the human uncoupling protein-2 gene, and juxtaposition to the uncoupling protein-3 gene

被引：49

作者：

Pecqueur, C ^{[1
]}

Cassard-Doulcier, AM ^{[1
]}

Raimbault, S ^{[1
]}

Miroux, B ^{[1
]}

Fleury, C ^{[1
]}

Gelly, C ^{[1
]}

Bouillaud, F ^{[1
]}

Ricquier, D ^{[1
]}

机构：

[1] CNRS, Ctr Rech Endocrinol Mol & Dev, UPR 9078, F-92190 Meudon, France

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 255卷 / 01期

关键词：

D O I：

10.1006/bbrc.1998.0146

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human and mouse UCP2 genes were cloned and sequenced. Transcriptional start sites were identified using primer extension analysis. The transcription unit of UCP2 gene is made of 2 untranslated exons followed by 6 exons encoding UCP2. In vitro translation analysis demonstrated that an open-reading-frame for a putative peptide of 36 residues present in exon 2 did not prevent UCP2 translation and confirmed that the initiation site of translation was in exon 3 as predicted from sequencing data. Short (bp -125 to +93) and long (bp -1383 and +93) CAT-constructs containing DNA upstream of the transcriptional start site of the human gene were made and transfected in adipocytes or HeLa cells allowing characterization of a potent promoter. Analysis of several genomic clones encompassing UCP2 and/or UCP3 genes demonstrated that the 2 genes are adjacent, the human UCP2 gene being located 7 kb downstream of the UCP3 gene. (C) 1999 Academic Press.

引用

页码：40 / 46

页数：7

共 36 条

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