A uniform system for microRNA annotation

被引：1392

作者：

Ambros, V

Bartel, B

Bartel, DP

Burge, CB

Carrington, JC

Chen, XM

Dreyfuss, G

Eddy, SR

Griffiths-Jones, S

Marshall, M

Matzke, M

Ruvkun, G

Tuschl, T

机构：

[1] Dartmouth Coll Sch Med, Dept Genet, Hanover, NH 03755 USA

[2] Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77005 USA

[3] MIT, Dept Biol, Cambridge, MA 02139 USA

[4] Whitehead Inst Biomed Res, Cambridge, MA 02139 USA

[5] Rutgers State Univ, Waksman Inst, Piscataway, NJ 08854 USA

[6] Univ Penn, Sch Med, Howard Hughes Med Inst, Philadelphia, PA 19104 USA

[7] Univ Penn, Sch Med, Dept Biochem & Biophys, Philadelphia, PA 19104 USA

[8] Washington Univ, Howard Hughes Med Inst, Dept Genet, St Louis, MO 63110 USA

[9] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England

[10] Austrian Acad Sci, Inst Mol Biol, A-5020 Salzburg, Austria

[11] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02114 USA

[12] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA

[13] Max Planck Inst Biophys Chem, Dept Cellular Biochem, Gottingen, Germany

[14] MIT, Dept Biol, Cambridge, MA 02142 USA

来源：

RNA | 2003年 / 9卷 / 03期

关键词：

microRNA; noncoding RNA; Rfam; Dicer; genome annotation;

D O I：

10.1261/rna.2183803

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs (miRNAs) are small noncoding RNA gene products about 22 nt long that are processed by Dicer from precursors with a characteristic hairpin secondary structure. Guidelines are presented for the identification and annotation of new miRNAs from diverse organisms, particularly so that miRNAs can be reliably distinguished from other RNAs such as small interfering RNAs. We describe specific criteria for the experimental verification of miRNAs, and conventions for naming miRNAs and miRNA genes. Finally, an online clearinghouse for miRNA gene name assignments is provided by the Rfam database of RNA families.

引用

页码：277 / 279

页数：3

共 18 条

[1] Rfam: an RNA family database [J].