Simple and rapid analytical method for carbapenems using capillary zone electrophoresis

被引：28

作者：

Taniguchi, S

Hamase, K

Kinoshita, A

Zaitsu, K ^{[1
]}

机构：

[1] Kyushu Univ, Fac Pharmaceut Sci, Fukuoka 8128582, Japan

[2] Kyushu Welf Pens Hosp, Dept Pharm, Yahatanishi Ku, Kitakyushu, Fukuoka 8068501, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B | 1999年 / 727卷 / 1-2期

关键词：

carbapenems; beta-lactams;

D O I：

10.1016/S0378-4347(99)00005-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and rapid analytical method for carbapenems using high-performance capillary electrophoresis is described. All therapeutic carbapenem injections in Japan (imipenem, panipenem and meropenem) and four other beta-lactams (piperacillin, cefotiam, cefotaxime, latamoxef) were separated and determined with good repeatability in about 10 min using simple free zone capillary electrophoresis. The electrophoresis buffer was 100 mM phosphate buffer of pH 8.0, and a fused-silica capillary of 25 mu m I.D. and 47 cm length was adopted. The present method was successfully applied to monitor the degradation of carbapenems under various conditions (at various temperatures or in coexistence with other drugs prescribed in the case of methicillin-resistant Staphylococcus aureus). (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：219 / 225

页数：7

共 11 条

[1] FEASIBILITY STUDIES ON RADIATION STERILIZATION OF CEPHRADINE [J].