Bar coding objects with DNA

被引:11
作者
Cox, JPL [1 ]
机构
[1] Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England
关键词
D O I
10.1039/b101850g
中图分类号
O65 [分析化学];
学科分类号
070302 [分析化学]; 081704 [应用化学];
摘要
A bioanalytical scheme is described for bar coding objects with DNA. Several DNA templates of different lengths are mixed together and the mixture used to label the object of interest. To disclose the bar code, the DNA mixture is eluted from the object, amplified by the polymerase chain reaction with a universal pair of primers, and then the amplified templates resolved by agarose gel electrophoresis. In its present state, the bar coding scheme allows for over a million different labels. The reliability of the scheme has been demonstrated in a typical situation by labelling a letter with a template mixture (similar to1.5 fmol of DNA), sending the letter through the post and successfully reading the bar code on receipt of the letter.
引用
收藏
页码:545 / 547
页数:3
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