Transgenic maize plants expressing the Totivirus antifungal protein, KP4, are highly resistant to corn smut

被引：31

作者：

Allen, Aron ^{[1
]}

Islamovic, Emir ^{[2
]}

Kaur, Jagdeep ^{[1
]}

Gold, Scott ^{[2
]}

Shah, Dilip ^{[1
]}

Smith, Thomas J. ^{[1
]}

机构：

[1] Donald Danforth Plant Sci Ctr, St Louis, MO USA

[2] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA

来源：

PLANT BIOTECHNOLOGY JOURNAL | 2011年 / 9卷 / 08期

关键词：

KP4; Ustilago maydis; transgenic; ENCODED FUNGAL TOXIN; USTILAGO-MAYDIS VIRUS-P4; KILLER PROTEINS; GENE; PATHOGEN; WHEAT; TRANSFORMATION; DEFENSINS; GROWTH; HOST;

D O I：

10.1111/j.1467-7652.2011.00590.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The corn smut fungus, Ustilago maydis, is a global pathogen responsible for extensive agricultural losses. Control of corn smut using traditional breeding has met with limited success because natural resistance to U. maydis is organ specific and involves numerous maize genes. Here, we present a transgenic approach by constitutively expressing the Totivirus antifungal protein KP4, in maize. Transgenic maize plants expressed high levels of KP4 with no apparent negative impact on plant development and displayed robust resistance to U. maydis challenges to both the stem and ear tissues in the greenhouse. More broadly, these results demonstrate that a high level of organ independent fungal resistance can be afforded by transgenic expression of this family of antifungal proteins.

引用

页码：857 / 864

页数：8

共 37 条

[11] PURIFICATION AND MOLECULAR-PROPERTIES OF THE TOXIN CODED BY USTILAGO-MAYDIS VIRUS-P4 [J].