A magnetic resonance imaging based method for measurement of tissue iron concentration in liver arterially embolized with ferrimagnetic particles designed for magnetic hyperthermia treatment of tumors

Rabbit liver was loaded with ferrimagnetic particles of gamma -Fe-2 O-3 (designed for magnetic hyperthermia treatment of liver tumors) by injecting various doses of a suspension of the particles into the hepatic artery in vivo. Proton transverse relaxation rate (R-2) images of the livers in vivo, excised, and dissected were generated from a series of single spin-echo images. Mean R, values for samples of ferrimagnetic-particle-loaded liver dissected into approximate 1 cm cubes were found to linearly correlate with tissue iron concentration over the range from approximately 0.1 to at least 2.7 mg Fe/g dry tissue when measured at room temperature. Changing the temperature of ferrimagnetic-particle-loaded samples of liver from VC to 37degreesC had no observable effect on tissue R-2 values. However, a small but significant decrease in R-2 was found for control samples containing no ferrimagnetic material on raising the temperature from 1degreesC to 37degreesC. Both chemically measured iron concentrations and mean R-2 values for rabbit livers with implanted tumors tended to be higher than those measured for tumor-free liver. This study indicates that tissue R-2 measurement and imaging by nuclear magnetic resonance may have a useful role in magnetic hyperthermia therapy protocols for the treatment of liver cancer. (C) 2003 Elsevier Inc. All rights reserved.