Millisecond-timescale, genetically targeted optical control of neural activity

被引：3391

作者：

Boyden, ES

Zhang, F

Bamberg, E

Nagel, G

Deisseroth, K

机构：

[1] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[2] Max Planck Inst Biophys, Dept Biophys Chem, D-60438 Frankfurt, Germany

[3] Univ Frankfurt, Dept Biochem Chem & Pharmaceut, D-60439 Frankfurt, Germany

[4] Stanford Univ, Sch Med, Dept Psychiat & Behav Sci, Stanford, CA 94305 USA

来源：

NATURE NEUROSCIENCE | 2005年 / 8卷 / 09期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/nn1525

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Temporally precise, noninvasive control of activity in well-defined neuronal populations is a long-sought goal of systems neuroscience. We adapted for this purpose the naturally occurring algal protein Channelrhodopsin-2, a rapidly gated light-sensitive cation channel, by using lentiviral gene delivery in combination with high-speed optical switching to photostimulate mammalian neurons. We demonstrate reliable, millisecond-timescale control of neuronal spiking, as well as control of excitatory and inhibitory synaptic transmission. This technology allows the use of light to alter neural processing at the level of single spikes and synaptic events, yielding a widely applicable tool for neuroscientists and biomedical engineers.

引用

收藏

页码：1263 / 1268

页数：6

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