Distinct roles of Fgf8, Foxi1, Dlx3b and Pax8/2 during otic vesicle induction and maintenance in medaka

被引:14
作者
Aghaallaei, Narges
Bajoghli, Baubak
Czerny, Thomas
机构
[1] Univ Vet Med, Inst Anim Breeding & Genet, A-1210 Vienna, Austria
[2] Univ Appl Sci, A-1030 Vienna, Austria
基金
奥地利科学基金会;
关键词
otic vesicle; inner ear; induction; dorsoventral patterning; heat-inducible system; medaka;
D O I
10.1016/j.ydbio.2007.04.022
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The development of the vertebrate inner ear is a complex process that has been investigated in several model organisms. In this work, we examined genetic interactions regulating early development of otic structures in medaka. We demonstrate that misexpression of Fgf8, Dlx3b and Foxi1 during early gastrulation is sufficient to produce ectopic otic vesicles. Combined misexpression strongly increases the appearance of this phenotype. By using a heat-inducible promoter we were furthermore able to separate the regulatory interactions among Fgf8, Foxi1, Dlx3b, Pax8 and Pax2 genes, which are active during different stages of early otic development. In the preplacodal stage we suggest a central position of Foxi1 within a regulatory network of early patterning genes including Dlx3b and Pax8. Different pathways are active after the placodal stage with Dlx3b playing a central role. There Dlx3b regulates members of the Pax-Six-Eya-Dach network and also strongly affects the early dorsoventral marker genes Otx1 and Gbx2. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:408 / 420
页数:13
相关论文
共 95 条
  • [71] A direct role for Fgf but not Wnt in otic placode induction
    Phillips, BT
    Storch, EM
    Lekven, AC
    Riley, BB
    [J]. DEVELOPMENT, 2004, 131 (04): : 923 - 931
  • [72] Zebrafish fgf3 and fgf8 encode redundant functions required for otic placode induction
    Phillips, BT
    Bolding, K
    Riley, BB
    [J]. DEVELOPMENTAL BIOLOGY, 2001, 235 (02) : 351 - 365
  • [73] FGF/FGFR-2(IIIb) signaling is essential for inner ear morphogenesis
    Pirvola, U
    Spencer-Dene, B
    Liang, XQ
    Kettunen, I
    Thesleff, I
    Fritzsch, B
    Dickson, C
    Ylikoski, J
    [J]. JOURNAL OF NEUROSCIENCE, 2000, 20 (16) : 6125 - 6134
  • [74] FGFR1 is required for the development of the auditory sensory epithelium
    Pirvola, U
    Ylikoski, J
    Trokovic, R
    Hébert, JM
    McConnell, SK
    Partanen, J
    [J]. NEURON, 2002, 35 (04) : 671 - 680
  • [75] Wnt-dependent regulation of inner ear morphogenesis is balanced by the opposing and supporting roles of Shh
    Riccomagno, MM
    Takada, S
    Epstein, DJ
    [J]. GENES & DEVELOPMENT, 2005, 19 (13) : 1612 - 1623
  • [76] Specification of the mammalian cochlea is dependent on Sonic hedgehog
    Riccomagno, MM
    Martinu, L
    Mulheisen, M
    Wu, DK
    Epstein, DJ
    [J]. GENES & DEVELOPMENT, 2002, 16 (18) : 2365 - 2378
  • [77] Ringing in the new ear: resolution of cell interactions in otic development
    Riley, BB
    Phillips, BT
    [J]. DEVELOPMENTAL BIOLOGY, 2003, 261 (02) : 289 - 312
  • [78] Dlx5 and Dlx6 homeobox genes are required for specification of the mammalian vestibular apparatus
    Robledo, Raymond F.
    Lufkin, Thomas
    [J]. GENESIS, 2006, 44 (09) : 425 - 437
  • [79] Induction and specification of cranial placodes
    Schlosser, Gerhard
    [J]. DEVELOPMENTAL BIOLOGY, 2006, 294 (02) : 303 - 351
  • [80] Genetic interactions underlying otic placode induction and formation
    Solomon, KS
    Kwak, SJ
    Fritz, A
    [J]. DEVELOPMENTAL DYNAMICS, 2004, 230 (03) : 419 - 433