Metabolic and metagenomic outcomes from early-life pulsed antibiotic treatment

被引:291
作者
Nobel, Yael R. [1 ]
Cox, Laura M. [1 ,2 ]
Kirigin, Francis F. [1 ]
Bokulich, Nicholas A. [1 ]
Yamanishi, Shingo [1 ]
Teitler, Isabel [1 ]
Chung, Jennifer [1 ]
Sohn, Jiho [1 ]
Barber, Cecily M. [1 ]
Goldfarb, David S. [1 ,3 ]
Raju, Kartik [1 ]
Abubucker, Sahar [4 ]
Zhou, Yanjiao [4 ,5 ,9 ]
Ruiz, Victoria E. [1 ]
Li, Huilin [6 ]
Mitreva, Makedonka [4 ,7 ]
Alekseyenko, Alexander V. [1 ,8 ]
Weinstock, George M. [4 ,9 ]
Sodergren, Erica [4 ,9 ]
Blaser, Martin J. [1 ,2 ,3 ]
机构
[1] NYU, Sch Med, Dept Med, New York, NY 10016 USA
[2] NYU, Sch Med, Dept Microbiol, New York, NY 10016 USA
[3] New York Harbor Dept Vet Affairs Med Ctr, New York, NY 10010 USA
[4] Washington Univ, Genome Inst, St Louis, MO 63108 USA
[5] Washington Univ, Sch Med, Dept Pediat, St Louis, MO 63110 USA
[6] NYU, Langone Med Ctr, Dept Populat Hlth, Div Biostat, New York, NY 10016 USA
[7] Washington Univ, Sch Med, Dept Internal Med, Div Infect Dis, St Louis, MO 63110 USA
[8] NYU, Sch Med, Ctr Hlth Informat & Bioinformat, New York, NY 10016 USA
[9] Jackson Lab Genom Med, Farmington, CT 06117 USA
关键词
GUT MICROBIOTA; CHILDHOOD OVERWEIGHT; AMOXICILLIN; PHARMACOKINETICS; COLONIZATION; ENVIRONMENT; ASSOCIATION; ALIGNMENT; EXPOSURE; WEIGHT;
D O I
10.1038/ncomms8486
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Mammalian species have co-evolved with intestinal microbial communities that can shape development and adapt to environmental changes, including antibiotic perturbation or nutrient flux. In humans, especially children, microbiota disruption is common, yet the dynamic microbiome recovery from early-life antibiotics is still uncharacterized. Here we use a mouse model mimicking paediatric antibiotic use and find that therapeutic-dose pulsed antibiotic treatment (PAT) with a beta-lactam or macrolide alters both host and microbiota development. Early-life PAT accelerates total mass and bone growth, and causes progressive changes in gut microbiome diversity, population structure and metagenomic content, with microbiome effects dependent on the number of courses and class of antibiotic. Whereas control microbiota rapidly adapts to a change in diet, PAT slows the ecological progression, with delays lasting several months with previous macrolide exposure. This study identifies key markers of disturbance and recovery, which may help provide therapeutic targets for microbiota restoration following antibiotic treatment.
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页数:15
相关论文
共 70 条
  • [21] Altering the Intestinal Microbiota during a Critical Developmental Window Has Lasting Metabolic Consequences
    Cox, Laura M.
    Yamanishi, Shingo
    Sohn, Jiho
    Alekseyenko, Alexander V.
    Leung, Jacqueline M.
    Cho, Ilseung
    Kim, Sungheon G.
    Li, Huilin
    Gao, Zhan
    Mahana, Douglas
    Rodriguez, Jorge G. Zarate
    Rogers, Arlin B.
    Robine, Nicolas
    Loke, P'ng
    Blaser, Martin J.
    [J]. CELL, 2014, 158 (04) : 705 - 721
  • [22] Pathways in Microbe-Induced Obesity
    Cox, Laura M.
    Blaser, Martin J.
    [J]. CELL METABOLISM, 2013, 17 (06) : 883 - 894
  • [23] Diet rapidly and reproducibly alters the human gut microbiome
    David, Lawrence A.
    Maurice, Corinne F.
    Carmody, Rachel N.
    Gootenberg, David B.
    Button, Julie E.
    Wolfe, Benjamin E.
    Ling, Alisha V.
    Devlin, A. Sloan
    Varma, Yug
    Fischbach, Michael A.
    Biddinger, Sudha B.
    Dutton, Rachel J.
    Turnbaugh, Peter J.
    [J]. NATURE, 2014, 505 (7484) : 559 - +
  • [24] Davis C., 2015, J Data Mining Genomics Proteomics, V4, DOI [DOI 10.4172/2153-0602.1000135, 10.4172/2153-0602.1000135]
  • [25] Infectious Diseases Society of America and the Society for Healthcare Epidemiology of America guidelines for developing an institutional program to enhance antimicrobial stewardship
    Dellit, Timothy H.
    Owens, Robert C.
    McGowan, John E., Jr.
    Gerding, Dale N.
    Weinstein, Robert A.
    Burke, John P.
    Huskins, W. Charles
    Paterson, David L.
    Fishman, Neil O.
    Carpenter, Christopher F.
    Brennan, P. J.
    Billeter, Marianne
    Hooton, Thomas M.
    [J]. CLINICAL INFECTIOUS DISEASES, 2007, 44 (02) : 159 - 177
  • [26] Antibiotic growth promoters in agriculture: History and mode of action
    Dibner, JJ
    Richards, JD
    [J]. POULTRY SCIENCE, 2005, 84 (04) : 634 - 643
  • [27] The ade4 package: Implementing the duality diagram for ecologists
    Dray, Stephane
    Dufour, Anne-Beatrice
    [J]. JOURNAL OF STATISTICAL SOFTWARE, 2007, 22 (04): : 1 - 20
  • [28] A sensitive assay of amoxicillin in mouse serum and broncho-alveolar lavage fluid by liquid-liquid extraction and reversed-phase HPLC
    Du, XL
    Li, CH
    Sun, HK
    Nightingale, CH
    Nicolau, DP
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2005, 39 (3-4) : 648 - 652
  • [29] EFFECT OF ANTIBACTERIAL DRUGS ON WEIGHT OF MICE
    DUBOS, R
    COSTELLO, RL
    SCHAEDLER, RW
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1963, 117 (02) : 245 - &
  • [30] High-level colonisation of the human gut by Verrucomicrobia following broad-spectrum antibiotic treatment
    Dubourg, Gregory
    Lagier, Jean-Christophe
    Armougom, Fabrice
    Robert, Catherine
    Audoly, Gilles
    Papazian, Laurent
    Raoult, Didier
    [J]. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2013, 41 (02) : 149 - 155