miR-1236 down-regulates alpha-fetoprotein, thus causing PTEN accumulation, which inhibits the PI3K/Akt pathway and malignant phenotype in hepatoma cells

被引：65

作者：

Gao, Rui ^{[1
,2
]}

Cai, Chunli ^{[1
,2
]}

Gan, Jiancheng ^{[3
]}

Yang, Xi ^{[1
,2
]}

Shuang, Zeyu ^{[4
]}

Liu, Min ^{[1
,2
]}

Li, Shengping ^{[4
]}

Tang, Hua ^{[1
,2
]}

机构：

[1] Tianjin Med Univ, Tianjin Life Sci Res Ctr, Tianjin, Peoples R China

[2] Tianjin Med Univ, Basic Med Sch, Tianjin, Peoples R China

[3] Tianjin Med Univ, Dept Surg, Secondary Hosp, Tianjin, Peoples R China

[4] Sun Yat Sen Univ, Dept Hepatobiliary Oncol, Ctr Canc, State Key Lab Oncol Southern China, Guangzhou 510275, Guangdong, Peoples R China

来源：

ONCOTARGET | 2015年 / 6卷 / 08期

基金：

中国国家自然科学基金;

关键词：

microRNA; microRNA-1236; AFP; PTEN; hepatocellular cancer; HUMAN HEPATOCELLULAR-CARCINOMA; X-LINKED INHIBITOR; SIGNALING PATHWAY; MESENCHYMAL TRANSITION; LIVER-CANCER; GENE; TARGETS; PROLIFERATION; PROGNOSIS; MARKER;

D O I：

10.18632/oncotarget.3338

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Alpha fetoprotein (AFP) is a clinical biomarker of hepatocellular carcinoma (HCC). Here, we found that miR-1236 is down-regulated, whereas AFP is highly expressed in HCC tissues and cells. We demonstrated that miR-1236 directly targets the 3'UTR of AFP and down-regulates its expression. Also, miR-1236 inhibited and AFP stimulated proliferation, migration, invasion and vasculogenic mimicry (VM) of HCC. In agreement, AFP over-expression counteracted the inhibitory effect of miR-1236. We demonstrated that AFP promoted the ubiquitination of PTEN, thus decreasing PTEN levels, while miR-1236 inhibited the PI3K/Akt pathway.

引用

页码：6014 / 6028

页数：15

共 41 条

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