Overexpression of a family of RPEL proteins modifies cell shape

被引：17

作者：

Favot, L ^{[1
]}

Gillingwater, M ^{[1
]}

Scott, C ^{[1
]}

Kemp, PR ^{[1
]}

机构：

[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England

来源：

FEBS LETTERS | 2005年 / 579卷 / 01期

关键词：

actin; cytoskeleton; cell shape; RPEL; subcellular localization;

D O I：

10.1016/j.febslet.2004.11.054

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins containing RPEL motifs (e.g., MAL) are important in the regulation of gene expression by the actin cytoskeleton. Screening the ENSEMBL database for RPEL proteins identified four additional proteins that contain RPEL motifs and nuclear localisation sequences, three of which (RPEL-A, RPEL-B and RPEL-C) are expressed in adult mouse tissues with different expression profiles. The mRNAs encoding RPEL-B and RPEL-C were subject to alternative splicing. Expression of these genes in cells indicated that they had a marked effect on cell shape. Furthermore, when expressed with a nuclear localised actin all of the different forms became restricted to the nucleus. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：100 / 104

页数：5

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