Heterologous Kluyveromyces lactis β-galactosidase secretion by Saccharomyces cerevisiae super-secreting mutants

被引：13

作者：

Becerra, M ^{[1
]}

Prado, SD ^{[1
]}

Cerdán, E ^{[1
]}

Siso, MIG ^{[1
]}

机构：

[1] Fac Ciencias, Dept Biol Celular & Mol, A Coruna 15071, Spain

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 01期

关键词：

beta-galactosidase; Kluyveromyces lactis; protein secretion; super-secreting mutants; yeast;

D O I：

10.1023/A:1026795706520

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Several Saccharomyces cerevisiae strains with a super-secreting phenotype have been transformed using a secretion plasmid containing the LAC4 gene and have proven to be effective in the secretion of Kluyveromyces lactis beta -galactosidase. The strain CGY1585 (ssc1-1) showed the highest secretion (1.7 EU ml(-1)) in the culture medium. As far as we know, Kluyveromyces lactis beta -galactosidase is the largest sized protein and the only intracellular one among those secreted by these mutants hitherto. The recombinant strains all grew in lactose media.

引用

页码：33 / 40

页数：8

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