Detection of anammox activity and diversity of anammox bacteria-related 16S rRNA genes in coastal marine sediment in japan

被引:124
作者
Amano, Teruki
Yoshinaga, Ikuo [1 ]
Okada, Kentaro
Yamagishi, Takao
Ueda, Shingo
Obuchi, Akira
Sako, Yoshihiko
Suwa, Yuichi
机构
[1] Kyoto Univ, Grad Sch Agr, Div Appl Sci, Lab Marine Microbiol,Sakyo Ku, Kyoto 6068502, Japan
[2] Natl Inst Adv Ind Sci & Technol, Res Inst Envronm Management Technol, Tsukuba, Ibaraki 3058569, Japan
[3] Nihon Univ, Coll Bioresource Sci, Fujisawa, Kanagawa 2528510, Japan
关键词
anammox; 16S rRNA gene; nitrogen cycle; marine sediment; N-15; tracer;
D O I
10.1264/jsme2.22.232
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A first assessment of anammox activity, which is a unique N-2 emission process, was conducted in samples of coastal marine sediment from Japan with a N-15 tracer. The occurrence and diversity of bacteria possibly responsible for the anammox process were also evaluated by selective PCR-amplification of the 16S rRNA gene for known anammox bacteria. Anammox activity, detected by measuring (NN)-N-14-N-15 gas production, was only found in samples collected at the intertidal sand bank located at the Yodo River estuary. In the Yodo River samples, 16S rRNA gene fragments affiliated with the known anammox bacterial lineage were also recovered, and the two major phylotypes were both "Candidatus Scalindua wagneri" relatives with 95% and 98% sequence similarity. Even from the other samples in which no recognizable anammox activity was detected, 16S rRNA gene fragments related to known anammox bacteria, but not to "Ca. S. wagneri", were detected. This is the first report of anammox-mediated N-2 emission in coastal marine environments in Japan. Notably, the PCR-based analysis allowed us to discover unexpected phylogenetic diversity of anammox bacteria-related 16S rRNA gene sequences. The selective PCR primer set developed in this study could be a powerful tool to unveil the ecology of anammox bacteria in natural environments.
引用
收藏
页码:232 / 242
页数:11
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