Measuring apoptosis at the single cell level

被引：59

作者：

Bouchier-Hayes, Lisa ^{[1
]}

Munoz-Pinedo, Cristina ^{[2
]}

Connell, Samuel ^{[1
]}

Green, Douglas R. ^{[1
]}

机构：

[1] St Jude Childrens Res Hosp, Dept Immunol, Memphis, TN 38105 USA

[2] Inst Invest Biomed Bellvitge IDIBELL, Barcelona 08907, Spain

来源：

METHODS | 2008年 / 44卷 / 03期

关键词：

apoptosis; mitochondria; time-lapse confocal microscopy; cytochrome c; caspase;

D O I：

10.1016/j.ymeth.2007.11.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of live cell microscopy has made a number of contributions to the study of apoptosis. Many of the tools and techniques are available that allow us to image the key events that occur during cell death including mitochondrial outer membrane permeabilization, mitochondrial transmembrane potential changes, translocation of Bcl-2 family members, caspase activation, phosphatidylserine flip and plasma membrane rupture. We discuss these techniques here and highlight the advantages and drawbacks of using such approaches to study apoptosis. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：222 / 228

页数：7

共 33 条

[1] Mitochondria: pharmacological manipulation of cell death [J].

Bouchier-Hayes, L ;

Lartigue, L ;

Newmeyer, DD .

JOURNAL OF CLINICAL INVESTIGATION, 2005, 115 (10) :2640-2647

[2] Mitochondrial membrane permeabilization and superoxide production during apoptosis -: A single-cell analysis [J].

Düssmann, H ;

Kögel, D ;

Rehm, M ;

Prehn, JHM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (15) :12645-12649

[3] Outer mitochondrial membrane permeabilization during apoptosis triggers caspase-independent mitochondrial and caspase-dependent plasma membrane potential depolarization:: a single-cell analysis [J].

Düssmann, H ;

Rehm, M ;

Kögel, D ;

Prehn, JHM .

JOURNAL OF CELL SCIENCE, 2003, 116 (03) :525-536

[4] Methods for the assessment of mitochondrial membrane permeabilization in apoptosis [J].

Galluzzi, Lorenzo ;

Zamzami, Naoufal ;

Rouge, Thibault de La Motte ;

Lemaire, Christophe ;

Brenner, Catherine ;

Kroemer, Guido .

APOPTOSIS, 2007, 12 (05) :803-813

[5] The coordinate release of cytochrome c during apoptosis is rapid, complete and kinetically invariant [J].

Goldstein, JC ;

Waterhouse, NJ ;

Juin, P ;

Evan, GI ;

Green, DR .

NATURE CELL BIOLOGY, 2000, 2 (03) :156-162

[6] Cytochrome c is released in a single step during apoptosis [J].

Goldstein, JC ;

Muñoz-Pinedo, C ;

Ricci, JE ;

Adams, SR ;

Kelekar, A ;

Schuler, M ;

Tsien, RY ;

Green, DR .

CELL DEATH AND DIFFERENTIATION, 2005, 12 (05) :453-462

[7] Specific covalent labeling of recombinant protein molecules inside live cells [J].

Griffin, BA ;

Adams, SR ;

Tsien, RY .

SCIENCE, 1998, 281 (5374) :269-272

[8] Mitochondrial depolarization accompanies cytochrome c release during apoptosis in PC6 cells [J].

Heiskanen, KM ;

Bhat, MB ;

Wang, HW ;

Ma, JJ ;

Nieminen, AL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (09) :5654-5658

[9] Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis [J].

Karbowski, M ;

Lee, YJ ;

Gaume, B ;

Jeong, SY ;

Frank, S ;

Nechushtan, A ;

Santel, A ;

Fuller, M ;

Smith, CL ;

Youle, RJ .

JOURNAL OF CELL BIOLOGY, 2002, 159 (06) :931-938

[10] Quantitation of mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis [J].

Karbowski, M ;

Arnoult, D ;

Chen, HC ;

Chan, DC ;

Smith, CL ;

Youle, RJ .

JOURNAL OF CELL BIOLOGY, 2004, 164 (04) :493-499

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