Functional photoacoustic microscopy of pH

被引：30

作者：

Chatni, Muhammad Rameez ^{[1
]}

Yao, Junjie ^{[1
]}

Danielli, Amos ^{[1
]}

Favazza, Christopher P. ^{[1
]}

Maslov, Konstantin I. ^{[1
]}

Wang, Lihong V. ^{[1
]}

机构：

[1] Washington Univ, Dept Biomed Engn, Opt Imaging Lab, St Louis, MO 63130 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2011年 / 16卷 / 10期

基金：

美国国家卫生研究院;

关键词：

photoacoustic microscopy; pH imaging; SNARF-5F carboxylic acid; RESOLUTION;

D O I：

10.1117/1.3644495

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

pH is a tightly regulated indicator of metabolic activity. In mammalian systems, an imbalance of pH regulation may result from or result in serious illness. In this paper, we report photoacoustic microscopy (PAM) of a commercially available pH-sensitive fluorescent dye (SNARF-5F carboxylic acid) in tissue phantoms. We demonstrated that PAM is capable of pH imaging in absolute values at tissue depths of up to 2.0 mm, greater than possible with other forms of optical microscopy. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3644495]

引用

页数：3

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