Cell type-specific genotoxic effects of intermittent extremely low-frequency electromagnetic fields

被引：80

作者：

Ivancsits, S ^{[1
]}

Pilger, A ^{[1
]}

Diem, E ^{[1
]}

Jahn, O ^{[1
]}

Rüdiger, HW ^{[1
]}

机构：

[1] Med Univ Vienna, Div Occupat Med, A-1090 Vienna, Austria

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2005年 / 583卷 / 02期

关键词：

ELF-EMF exposure; comet assay; different cell types;

D O I：

10.1016/j.mrgentox.2005.03.011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The issue of adverse health effects of extremely low-frequency electromagnetic fields (ELF-EMFs) is highly controversial. Contradictory results regarding the genotoxic potential of ELF-EMF have been reported in the literature, To test whether this controversy might reflect differences between the cellular targets examined we exposed Cultured cells derived from different tissues to an intermittent ELF-EMF (50 Hz sinusoidal, 1 mT) for 1-24 h. The alkaline and neutral comet assays were used to assess ELF-EMF-induced DNA strand breaks. We could identify three responder (human fibroblasts, human melanocytes, rat granulosa cells) and three non-responder cell types (human lymphocytes, human monocytes, human skeletal muscle cells), which points to the significance of the cell system used when investigating genotoxic effects of ELF-EMF (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：184 / 188

页数：5

共 29 条

[1] THE EFFECT OF VARIOUS ANTIOXIDANTS AND OTHER MODIFYING AGENTS ON OXYGEN-RADICAL-GENERATED DNA-DAMAGE IN HUMAN-LYMPHOCYTES IN THE COMET ASSAY [J].

ANDERSON, D ;

YU, TW ;

PHILLIPS, BJ ;

SCHMEZER, P .

MUTATION RESEARCH, 1994, 307 (01) :261-271

[2] Nonlinear cell response to strong electric fields [J].

Bardos, DC ;

Thompson, CJ ;

Yang, YS ;

Joyner, KH .

PHYSICS IN MEDICINE AND BIOLOGY, 2000, 45 (07) :1965-1988

[3] Basal levels of DNA strand breaks in human leukocytes determined by comet assay [J].

Diem, E ;

Ivancsits, S ;

Rüdiger, HW .

JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A, 2002, 65 (09) :641-648

[4] Vanadate induces DNA strand breaks in cultured human fibroblasts at doses relevant to occupational exposure [J].

Ivancsits, S ;

Pilger, A ;

Diem, E ;

Schaffer, A ;

Rüdiger, HW .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2002, 519 (1-2) :25-35

[5] Age-related effects on induction of DNA strand breaks by intermittent exposure to electromagnetic fields [J].

Ivancsits, S ;

Diem, E ;

Jahn, O ;

Rüdiger, HW .

MECHANISMS OF AGEING AND DEVELOPMENT, 2003, 124 (07) :847-850

[6] Intermittent extremely low frequency electromagnetic fields cause DNA damage in a dose-dependent way [J].

Ivancsits, S ;

Diem, E ;

Jahn, O ;

Rüdiger, HW .

INTERNATIONAL ARCHIVES OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH, 2003, 76 (06) :431-436

[7] Induction of DNA strand breaks by intermittent exposure to extremely-low-frequency electromagnetic fields in human diploid fibroblasts [J].

Ivancsits, S ;

Diem, E ;

Pilger, A ;

Rüdiger, HW ;

Jahn, O .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2002, 519 (1-2) :1-13

[8] Protective effect of melatonin against in vitro iron ions and 7 mT 50 Hz magnetic field-induced DNA damage in rat lymphocytes [J].

Jajte, J ;

Zmyslony, M ;

Palus, J ;

Dziubaltowska, E ;

Rajkowska, E .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2001, 483 (1-2) :57-64

[9] ESTABLISHMENT OF STEROIDOGENIC GRANULOSA-CELL LINES EXPRESSING FOLLICLE-STIMULATING-HORMONE RECEPTORS [J].

KERENTAL, I ;

DANTES, A ;

SPRENGEL, R ;

AMSTERDAM, A .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1993, 95 (1-2) :R1-R10

[10] CYTOGENETIC EFFECTS OF PULSING ELECTROMAGNETIC-FIELD ON HUMAN-LYMPHOCYTES INVITRO - CHROMOSOME-ABERRATIONS, SISTER-CHROMATID EXCHANGES AND CELL-KINETICS [J].

KHALIL, AM ;

QASSEM, W .

MUTATION RESEARCH, 1991, 247 (01) :141-146

← 1 2 3 →