Investigation of guanidine hydrochloride induced chlorophyll protein 43 and 47 denaturation in the terahertz frequency range

被引:17
作者
Chen, Hua
Wang, Li
Qu, Yuangang
Kuang, Tingyun
Li, Liangbi
Peng, Weixian
机构
[1] Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Lab Opt Phys, Beijing 100080, Peoples R China
[2] Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China
[3] Jilin Univ, Coll Phys, Changchun 130023, Peoples R China
基金
中国国家自然科学基金;
关键词
D O I
10.1063/1.2787956
中图分类号
O59 [应用物理学];
学科分类号
摘要
In investigating guanidine (Gu)HCl-denatured chlorophyll protein 43 (CP43) and CP47 using the terahertz time-domain spectroscopy, we explored the feasibility and sensitivity of the terahertz technology in sensing protein denaturation and associated conformation changes. It was found that the conformation change was induced by the C = O group of chlorophyll a, interacting with the N-H group of GuHCl to form hydrogen bonds. According to the fluorescence emission spectra of the CPs treated by GuHCl with different concentrations, we found that CP47 is more sensitive to GuHCl treatment than CP43. (C) 2007 American Institute of Physics.
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页数:3
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