Separation of Disaccharides by Comprehensive Two-Dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry. Application to Honey Analysis

被引：12

作者：

Brokl, Michal ^{[1
]}

Soria, Ana C. ^{[1
]}

Ruiz-Matute, Ana I. ^{[1
]}

Luz Sanz, Maria ^{[1
]}

Ramos, Lourdes ^{[1
]}

机构：

[1] CSIC, Inst Quim Organ Gen, E-28006 Madrid, Spain

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2010年 / 58卷 / 22期

关键词：

GCxGC-ToF MS; disaccharides; trimethylsilyl oximes; honey; ETHER OXIME DERIVATIVES; TRIMETHYLSILYL DERIVATIVES; QUANTITATIVE-DETERMINATION; GC-MS; X GC; TRISACCHARIDES; IDENTIFICATION; SUGARS; MONO;

D O I：

10.1021/jf102646n

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A new method based on comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC x GC-ToF MS) has been developed for the first time for the analysis of complex mixtures of disaccharides previously converted to their trimethylsilyl oximes (TMSO). Among the different experimental parameters considered for optimization, both the column set combination and the dimensions of the second-dimension column were found to be the most significant with regard to the complete resolution of structurally similar disaccharides. Application of the optimized method to honey analysis allowed the separation of most of the honey disaccharides previously described in the literature. Furthermore, 12 other unknown disaccharides have been separated by this method and characterized from their mass spectral data.

引用

页码：11561 / 11567

页数：7

共 21 条

[1] Recent developments in comprehensive two-dimensional gas chromatography (GC X GC) I. Introduction and instrumental set-up [J].