Identification of puffer fish species by native isoelectric focusing technique

被引：18

作者：

Chen, TY

Shiau, CY

Noguchi, T

Wei, CI

Hwang, DF ^{[1
]}

机构：

[1] Natl Taiwan Ocean Univ, Dept Food Sci, Chilung 202, Taiwan

[2] Oklahoma State Univ, Coll Human Environm Sci, Stillwater, OK 74078 USA

来源：

FOOD CHEMISTRY | 2003年 / 83卷 / 03期

关键词：

native IEF; puffer fish; species identification; electrophoresis; protein;

D O I：

10.1016/S0308-8146(03)00253-X

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study was designed to validate the feasibility of using native isoelectric focusing electrophoresis (IEF) of water-soluble sarcoplasmic proteins in identifying puffer fish species. Each of the six tested species of puffer fish Lagocephalus wheeleri, L. gloveri, L. lunaris, L. inermis, L. sceleratus, and Takifugu oblongus showed species-specific protein band profiles after the focused gel was treated with Coomassie blue or silver stain. The majority of water-soluble puffer fish muscle proteins fell in the region with isoelectric point (pI) values of 5.85-8.65. Characteristic species-specific protein bands were present in all the three regions of pI 3.50-5.20, pI 5.85-6.55, and pI 7.35-8.15. Therefore, the species of puffer fish can be differentiated from the comparison of the characteristic native IEF protein band profiles. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：475 / 479

页数：5

共 26 条

[1] THE USE OF HPLC PROTEIN PROFILES IN FISH SPECIES IDENTIFICATION [J].