Anhydrobiosis without trehalose in bdelloid rotifers

被引：122

作者：

Lapinski, J ^{[1
]}

Tunnacliffe, A ^{[1
]}

机构：

[1] Univ Cambridge, Inst Biotechnol, Cambridge CB2 1QT, England

来源：

FEBS LETTERS | 2003年 / 553卷 / 03期

关键词：

anhydrobiosis; trehalose; desiccation tolerance; bdelloid rotifer;

D O I：

10.1016/S0014-5793(03)01062-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eukaryotes able to withstand desiccation enter a state of suspended animation known as anhydrobiosis, which is thought to require accumulation of the non-reducing disaccharides trehalose (animals, fungi) and sucrose (plants), acting as water replacement molecules and vitrifying agents. We now show that clonal populations of bdelloid rotifers Philodina roseola and Adineta vaga exhibit excellent desiccation tolerance, but that trehalose and other disaccharides are absent from carbohydrate extracts of dried animals. Furthermore, trehalose synthase genes (tps) were not found in rotifer genomes. This first observation of animal anhydrobiosis without trehalose challenges our current understanding of the phenomenon and calls for a re-evaluation of existing models. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：387 / 390

页数：4

共 35 条

[11]

Cuming AC, 1999, SEED PROTEINS, P753

[12] Anhydrobiotic engineering - Reply [J].

不详 .

NATURE BIOTECHNOLOGY, 2000, 18 (05) :473-473

[13] Intracellular trehalose improves osmotolerance but not desiccation tolerance in mammalian cells [J].

de Castro, AG ;

Tunnacliffe, A .

FEBS LETTERS, 2000, 487 (02) :199-202

[14]

EVANS AAF, 1969, NEMATOLOGY, V2, P99

[15] THE ROLE OF TREHALOSE IN DEHYDRATION RESISTANCE OF SACCHAROMYCES-CEREVISIAE [J].

GADD, GM ;

CHALMERS, K ;

REED, RH .

FEMS MICROBIOLOGY LETTERS, 1987, 48 (1-2) :249-254

[16] Trehalose expression confers desiccation tolerance on human cells [J].

Guo, N ;

Puhlev, I ;

Brown, DR ;

Mansbridge, J ;

Levine, F .

NATURE BIOTECHNOLOGY, 2000, 18 (02) :168-171

[17] NEW INSIGHTS INTO THE ANHYDROBIOTIC PHENOMENON - THE EFFECTS OF TREHALOSE CONTENT AND DIFFERENTIAL RATES OF EVAPORATIVE WATER-LOSS ON THE SURVIVAL OF APHELENCHUS-AVENAE [J].

HIGA, LM ;

WOMERSLEY, CZ .

JOURNAL OF EXPERIMENTAL ZOOLOGY, 1993, 267 (02) :120-129

[18] Mechanisms of plant desiccation tolerance [J].

Hoekstra, FA ;

Golovina, EA ;

Buitink, J .

TRENDS IN PLANT SCIENCE, 2001, 6 (09) :431-438

[19] RAPID CHANGES OF HEAT AND DESICCATION TOLERANCE CORRELATED WITH CHANGES OF TREHALOSE CONTENT IN SACCHAROMYCES-CEREVISIAE CELLS SUBJECTED TO TEMPERATURE SHIFTS [J].

HOTTIGER, T ;

BOLLER, T ;

WIEMKEN, A .

FEBS LETTERS, 1987, 220 (01) :113-115

[20] MOLECULAR-CLONING AND PHYSICAL MAPPING OF THE OTSBA GENES, WHICH ENCODE THE OSMOREGULATORY TREHALOSE PATHWAY OF ESCHERICHIA-COLI - EVIDENCE THAT TRANSCRIPTION IS ACTIVATED BY KATF (APPR) [J].

KAASEN, I ;

FALKENBERG, P ;

STYRVOLD, OB ;

STROM, AR .

JOURNAL OF BACTERIOLOGY, 1992, 174 (03) :889-898

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