Mapping the Mouse Cell Atlas by Microwell-Seq

被引：1091

作者：

Han, Xiaoping ^{[1
,13
]}

Wang, Renying ^{[1
,13
]}

Zhou, Yincong ^{[2
,13
]}

Fei, Lijiang ^{[1
,13
]}

Sun, Huiyu ^{[1
,13
]}

Lai, Shujing ^{[1
,13
]}

Saadatpour, Assieh ^{[12
]}

Zhou, Zimin ^{[1
,13
]}

Chen, Haide ^{[1
,13
]}

Ye, Fang ^{[1
,13
]}

Huang, Daosheng ^{[1
]}

Xu, Yang ^{[1
]}

Huang, Wentao ^{[1
]}

Jiang, Mengmeng ^{[1
,13
]}

Jiang, Xinyi ^{[1
,13
]}

Mao, Jie ^{[3
]}

Chen, Yao ^{[4
]}

Lu, Chenyu ^{[5
]}

Xie, Jin ^{[6
]}

Fang, Qun ^{[7
]}

Wang, Yibin ^{[8
]}

Yue, Rui ^{[8
]}

Li, Tiefeng ^{[3
]}

Huang, He ^{[9
,13
]}

Orkin, Stuart H. ^{[10
,11
]}

Yuan, Guo-Cheng ^{[12
]}

Chen, Ming ^{[2
,13
]}

Guo, Guoji ^{[1
,9
,13
]}

机构：

[1] Zhejiang Univ, Ctr Stem Cell & Regenerat Med, Sch Med, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Univ, Coll Life Sci, Hangzhou 310003, Zhejiang, Peoples R China

[3] Zhejiang Univ, Inst Appl Mech, Hangzhou 310027, Zhejiang, Peoples R China

[4] Zhejiang Univ, Womens Hosp, Sch Med, Dept Reprod Endocrinol, Hangzhou 310058, Zhejiang, Peoples R China

[5] Zhejiang Univ, Sch Med, Res Ctr Infect & Immun, Hangzhou 310058, Zhejiang, Peoples R China

[6] Zhejiang Univ, State Key Lab Fluid Power & Mechatron Syst, Hangzhou 310058, Zhejiang, Peoples R China

[7] Zhejiang Univ, Inst Microanalyt Syst, Dept Chem, Hangzhou 310058, Zhejiang, Peoples R China

[8] Tongji Univ, Shanghai East Hosp, Sch Life Sci & Technol, Inst Regenerat Med, Shanghai 200092, Peoples R China

[9] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Inst Hematol, Hangzhou 310003, Zhejiang, Peoples R China

[10] Harvard Med Sch, Dana Farber Canc Inst, Div Pediat Hematol Oncol, Boston, MA 02115 USA

[11] Harvard Med Sch, Boston Childrens Hosp, Boston, MA 02115 USA

[12] Harvard Sch Publ Hlth, Dana Farber Canc Inst, Dept Biostat & Computat Biol, Boston, MA 02115 USA

[13] Zhejiang Univ, Stem Cell Inst, Hangzhou 310058, Zhejiang, Peoples R China

来源：

CELL | 2018年 / 172卷 / 05期

基金：

中国国家自然科学基金;

关键词：

MESSENGER-RNA-SEQ; GENE-EXPRESSION; CHROMATIN ACCESSIBILITY; SINGLE CELLS; DIFFERENTIATION; IDENTIFICATION; METHYLATION; VARIABILITY; EVOLUTION; IMMUNE;

D O I：

10.1016/j.cell.2018.02.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-cell RNA sequencing (scRNA-seq) technologies are poised to reshape the current cell-type classification system. However, a transcriptome-based single-cell atlas has not been achieved for complex mammalian systems. Here, we developed Microwell-seq, a high-throughput and low-cost scRNA-seq platform using simple, inexpensive devices. Using Microwell-seq, we analyzed more than 400,000 single cells covering all of the major mouse organs and constructed a basic scheme for a mouse cell atlas (MCA). We reveal a single-cell hierarchy for many tissues that have not been well characterized previously. We built a web-based "single-cell MCA analysis" pipeline that accurately defines cell types based on single-cell digital expression. Our study demonstrates the wide applicability of the Microwell-seq technology and MCA resource.

引用

页码：1091 / +

页数：34

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