Enzymatic biosensors based on carbon nanotubes paste electrodes

被引：121

作者：

Rubianes, MD ^{[1
]}

Rivas, GA ^{[1
]}

机构：

[1] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Quim Fis, INFIQC, RA-5000 Cordoba, Argentina

来源：

ELECTROANALYSIS | 2005年 / 17卷 / 01期

关键词：

carbon nanotubes; composite; biosensor; lactate oxidase; lactate; polyphenol oxidase; phenols; catechols; alcohol dehydrogenase; NADH; alcohols; catalysis;

D O I：

10.1002/elan.200403121

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The performance of enzymatic biosensors based on the immobilization of different enzymes within a carbon nanotubes paste electrode (CNTPE) prepared by dispersion of multi-wall carbon nanotubes (MWNT) and mineral oil is reported in this work. The strong electrocatalytic activity of carbon nanotubes towards the reduction of hydrogen peroxide and quinones and the oxidation of NADH have allowed an effective low-potential amperometric determination of lactate, phenols, catechols and ethanol, in connection with the incorporation of lactate oxidase, polyphenol oxidase and alcohol dehydrogenase/NAD(+), respectively, within the composite matrix. Compared to the analogous enzymatic CPEs, a great enhancement in the response is observed at the enzymatic CNTPEs. Therefore, highly sensitive lactate, phenols, catechols and alcohols biosensors without using any metal or redox mediator can be obtained with this new composite material.

引用

页码：73 / 78

页数：6

共 24 条

[1] A comparison between the use of a redox mediator in solution and of surface modified electrodes in the electrocatalytic oxidation of nicotinamide adenine dinucleotide [J].

Antiochia, R ;

Lavagnini, I ;

Pastore, P ;

Magno, F .

BIOELECTROCHEMISTRY, 2004, 64 (02) :157-163

[2] Carbon nanotubes - the route toward applications [J].

Baughman, RH ;

Zakhidov, AA ;

de Heer, WA .

SCIENCE, 2002, 297 (5582) :787-792

[3] Carbon nanotube electrode for oxidation of dopamine [J].

Britto, PJ ;

Santhanam, KSV ;

Ajayan, PM .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1996, 41 (01) :121-125

[4] BIOCATALYSIS WITH POLYPHENOL OXIDASE - A REVIEW [J].

BURTON, SG .

CATALYSIS TODAY, 1994, 22 (03) :459-487

[5] Nanotubes, nanoscience, and nanotechnology [J].

Cohen, ML .

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 2001, 15 (1-2) :1-11

[6] Protein electrochemistry at carbon nanotube electrodes [J].

Davis, JJ ;

Coles, RJ ;

Hill, HAO .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1997, 440 (1-2) :279-282

[7] HELICAL MICROTUBULES OF GRAPHITIC CARBON [J].

IIJIMA, S .

NATURE, 1991, 354 (6348) :56-58

[8] Fabrication of carbon nanotubes [J].

Kingston, CT ;

Simard, B .

ANALYTICAL LETTERS, 2003, 36 (15) :3119-3145

[9] Detection of homocysteine at carbon nanotube paste electrodes [J].

Lawrence, NS ;

Deo, RP ;

Wang, J .

TALANTA, 2004, 63 (02) :443-449

[10] Low-potential stable NADH detection at carbon-nanotube-modified glassy carbon electrodes [J].

Musameh, M ;

Wang, J ;

Merkoci, A ;

Lin, YH .

ELECTROCHEMISTRY COMMUNICATIONS, 2002, 4 (10) :743-746

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