Efficient synthesis of O-linked glycopeptide by a transglycosylation using endo α-N-acetylgalactosaminidase from Streptomyces sp.

被引：10

作者：

Ajisaka, K

Miyasato, M

Ishii-Karakasa, I

机构：

[1] Meiji Milk Prod Co Ltd, Nutrit Sci Inst, Odawara, Kanagawa 2500862, Japan

[2] Kitasato Univ, Sch Med, Dept Biochem, Sagamihara, Kanagawa 2288555, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2001年 / 65卷 / 05期

关键词：

glycopeptide synthesis; mucin-type; endo-alpha-N-acetylgalactosaminidase; transglycosylation;

D O I：

10.1271/bbb.65.1240

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

GaI beta-(1 -->3)-Ga1NAc-linked hexapeptide was synthesized by a transglycosylation using GaI beta-(1 -->3)-Gal-NAc beta -pNP as a donor and a serine-containing hexapeptide as an acceptor using endo GaINAc-ase from Streptomyces sp.. The GaI beta-(1 -->3)-GaINAc residue was transferred to the hydroxyl group of the serine residue of the peptide. The total yield of the glycopeptide via this process,vas better than that of the chemoenzymatic method. This profess was confirmed to be a versatile method for the synthesis of O-linked glycopeptides.

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页码：1240 / 1243

页数：4

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