Purification and properties of recombinant β-galactosidase from Bacillus circulans

被引：75

作者：

Fujimoto, H ^{[1
]}

Miyasato, M

Ito, Y

Sasaki, T

Ajisaka, K

机构：

[1] Meiji Inst Hlth Sci, Naruda Odawara 250, Japan

[2] Meiji Cent Res Inst, Naruda Odawara 250, Japan

来源：

GLYCOCONJUGATE JOURNAL | 1998年 / 15卷 / 02期

关键词：

beta-galactosidase; Bacillus circulans;

D O I：

10.1023/A:1006916222187

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A gene encoding beta-galactosidase from Bacillus circulans which had hydrolysis specificity for the beta 1-3 linkage was expressed in Escherichia coli. The beta-galactosidase was purified from crude cell lysates of E. coli by column chromatographies on Resource Q and Sephacryl S-200 HR. The enzyme released galactose with high selectivity from oligosaccharides which had terminal beta 1-3 linked galactose residues. However it did not hydrolyse beta 1-4 linked galactooligosaccharides. Moreover, Gal beta 1-3GlcNAc, Gal beta 1-3GalNAc, and their p-nitrophenyl glycosides were regioselectively synthesized in 10-46% yield by the transglycosylation reaction using this enzyme.

引用

页码：155 / 160

页数：6

共 14 条

[1] REGIOSELECTIVE TRANSGLYCOSYLATION IN THE SYNTHESIS OF OLIGOSACCHARIDES - COMPARISON OF BETA-GALACTOSIDASES AND SIALIDASES OF VARIOUS ORIGINS [J].