Efficient synthesis of a sialyl T-antigen-linked glycopeptide by the chemoenzymatic method

被引：12

作者：

Ajisaka, K ^{[1
]}

Miyasato, M ^{[1
]}

机构：

[1] Meiji Milk Prod Co Ltd, Meiji Inst Hlth Sci, Odawara 2500862, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2000年 / 64卷 / 08期

关键词：

peptide synthesis; glycopeptide; mucin-type; beta-galactosidase; sialyltransferase;

D O I：

10.1271/bbb.64.1743

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sialyl T-antigen-linked tetrapeptide was prepared by the combined method of chemical synthesis and enzymatic synthesis. The GalNAc-linked peptide was first obtained bg using a commercial peptide synthesizer, and then a galactose residue was attached with beta-(1 --> 3)-linkage by transglycosylating with a recombinant beta-galactosidase from Bacillus circulans. The sialic acid residue was then combined by alpha-(2 --> 3)-linkage with sialytransferase from rat liver.

引用

页码：1743 / 1746

页数：4

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