Design of manganese porphyrin modified with mitochondrial signal peptide for a new antioxidant

被引：48

作者：

Asayama, Shoichiro ^{[1
]}

Kawamura, Eri ^{[1
]}

Nagaoka, Shoji ^{[1
]}

Kawakami, Hiroyoshi ^{[1
]}

机构：

[1] Tokyo Metropolitan Univ, Dept Appl Chem, Tokyo 1920397, Japan

来源：

MOLECULAR PHARMACEUTICS | 2006年 / 3卷 / 04期

基金：

日本学术振兴会;

关键词：

water-soluble manganese porphyrin; superoxide dismutase activity; signal peptide; mitochondrion; organella targeting;

D O I：

10.1021/mp0500667

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A new design of antioxidant, consisting of manganese (Mn) porphyrin and signal peptide for mitochondrial targeting, is reported. The resulting Mn-porphyrin-oligopeptide conjugate exhibited significant superoxide dismutase (SOD) activity and decomposed peroxynitrite (ONOO-). The new antioxidant caused the swelling of isolated mitochondria. By using the pH-sensitive drug carrier for intracellular delivery, furthermore, the new conjugate recovered the viability of lipopolysaccharide (LPS)-stimulated macrophage RAW 264.7 cells. These results suggest that the Mn-porphyrin modified with signal peptide for mitochondrial targeting is promising for a new class of antioxidants.

引用

页码：468 / 470

页数：3

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