Structural analysis of a new glycosphingolipid from the lipopolysaccharide-lacking bacterium Sphingomonas adhaesiva

被引：22

作者：

Kawahara, K

Lindner, B

Isshiki, Y

Jakob, K

Knirel, YA

Zähringer, U

机构：

[1] Res Ctr, Div Immunochem, D-23845 Borstel, Germany

[2] Kitasato Inst, Dept Bacteriol, Tokyo 1088642, Japan

[3] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow 117913, Russia

来源：

CARBOHYDRATE RESEARCH | 2001年 / 333卷 / 01期

关键词：

Sphingomonas adhaesiva; glycosphingolipid; ceramide;

D O I：

10.1016/S0008-6215(01)00111-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A new glycosphingolipid, GSL-4B, was isolated from Sphingomonas adhaesiva and found to share the ceramide moiety with GSL-1 and GSL-3 from Sphingomonas capsulata studied earlier [Kawahara, K.; Moll, H.; Knirel, Y. A.; Seydel, U.; Zahringer, U. Eur. J. Biochem. 2000, 267, 1837-1846]. It is heterogeneous with respect to the long-chain bases erythro-2-amino-1,3-octadecanediol (sphinganine), (13Z)-erythro-2-amino-13-eicosene-1,3-diol, and (13Z)-ery-thro -2-amino-13, 14-methylene-1,3-eicosanediol which in GSL-4B are present ill the ratios of 1.1:1.0:1.1, and all bearing amide-linked (S)-2-hydroxymyristic acid. Methylation analysis and MALDI-TOF-MS along with H-1 and C-13 NMR spectroscopy showed that the carbohydrate part of GSL-4B has the structure of alpha -D-Glcp-(1 -->4)-alpha -D-Galp-(1 -->6)-alpha -D-Glcp-(1 -->4)-alpha -D-GlcpA-(1 -->1)-Cer (C) 2001 Published by Elsevier Science Ltd.

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页码：87 / 93

页数：7

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