Signal-enhancer molecules encapsulated liposome as a valuable sensing and amplification platform combining the aptasensor for ultrasensitive ECL immunoassay

被引:36
作者
Mao, Li [1 ,2 ]
Yuan, Ruo [1 ]
Chai, Yaqin [1 ]
Zhuo, Ying [1 ]
Xiang, Yun [1 ]
机构
[1] Southwest Univ, Coll Chem & Chem Engn, Educ Minist, Key Lab Luminescence & Real Time Anal, Chongqing 400715, Peoples R China
[2] China W Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Nanchong 637002, Peoples R China
关键词
Liposome; ECL aptasensor; Signal amplification; NT-proBNP; Cocaine; NT-PROBNP ASSAY; ROLLING-CIRCLE AMPLIFICATION; BRAIN NATRIURETIC PEPTIDE; ELECTROGENERATED CHEMILUMINESCENCE; CHOLERA-TOXIN; APTAMER; COCAINE; SENSOR; COMPLEX; IMMUNOLIPOSOME;
D O I
10.1016/j.bios.2011.02.035
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
An innovatory ECL immunoassay strategy was proposed to detect the newly developing heart failure biomarker N-terminal pro-brain natriuretic peptide (NT-proBNP). Firstly, this strategy used small molecules encapsulated liposome as immune label to construct a sandwich immune sensing platform for NT-proBNP. Then the ECL aptasensor was prepared to collect and detect the small molecules released from the liposome. Finally, based on the ECL signal changes caused by the small molecules, the ECL signal indirectly reflected the level of NT-proBNP antigen. In this experiment, the cocaine was chosen as the proper small molecule that can act as signal-enhancer to enhance the ECL of Ru(bpy)(3)(2+). The cocaine-encapsulated liposomes were successfully characterized by TEM. The quantificational calculation proved the similar to 5.3 x 10(3) cocaine molecules per liposome enough to perform the assignment of signal amplification. The cocaine-binding ECL aptasensor further promoted the work aimed at amplifying signal. The performance of NT-proBNP assay by the proposed strategy exhibited high sensitivity and high specificities with a linear relationship over 0.01-500 ng mL(-1) range, and a detection limit down to 0.77 pg mL(-1). (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:4204 / 4208
页数:5
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