An Improved PCR-RFLP Assay for the Detection of a Polymorphism rs2289487 of PLIN1 Gene

被引：9

作者：

Feng, Xiaolei ^{[1
]}

Wang, Sihua ^{[1
]}

Duan, Xiaoran ^{[2
]}

Li, Chunyang ^{[3
]}

Yan, Zhen ^{[1
]}

Feng, Feifei ^{[3
]}

Yu, Songcheng ^{[4
]}

Wu, Yongjun ^{[3
]}

Wang, Wei ^{[1
]}

机构：

[1] Zhengzhou Univ, Coll Publ Hlth, Dept Occupat Hlth & Occupat Dis, 100 Kexue Rd, Zhengzhou 450001, Peoples R China

[2] Zhengzhou Univ, Dept Environm Hlth, Coll Publ Hlth, Zhengzhou 450001, Peoples R China

[3] Zhengzhou Univ, Dept Toxicol, Coll Publ Hlth, Zhengzhou 450001, Peoples R China

[4] Zhengzhou Univ, Dept Sanit Chem, Coll Publ Hlth, Zhengzhou 450001, Peoples R China

来源：

JOURNAL OF CLINICAL LABORATORY ANALYSIS | 2016年 / 30卷 / 06期

基金：

中国国家自然科学基金;

关键词：

PCR-RFLP; created restriction site; single-nucleotide polymorphism; rs2289487; PLIN1; PERILIPIN GENE; OBESITY RISK; SUSCEPTIBILITY; LOCUS; WOMEN; ASSOCIATION; POPULATION; HAPLOTYPE; 15Q;

D O I：

10.1002/jcla.21968

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

100118 [医学信息学]; 100208 [临床检验诊断学];

摘要：

BackgroundIn recent research, it has been shown that rs2289487 within the PLIN1 gene has different variants that have been associated with obesity, type 2 diabetes, and other diseases. However, the isochizomers such as the BsmI enzyme required for detection of this polymorphism through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method are expensive. In this study, we aimed to explore a novel PCR-RFLP method for identifying the single-nucleotide polymorphism (SNP) of rs2289487 of PLIN1 gene. MethodsA new restriction enzyme site was created through created restriction site PCR. In the forward primer, a deoxynucleotide A was substituted with C, and after PCR a new restriction enzyme site for BstUI was introduced into the PCR products. A total of 108 samples from Han Chinese were tested to evaluate this new method. ResultsAllele frequencies in the Asian population were 0.326 for allele A and 0.674 for allele G, and the genotype frequencies were 12.8% for AA, 39.5% for AG, and 47.7% for GG. ConclusionThe PCR-RFLP with new site for detecting the SNP of rs2289487 is an improved method with low cost and high accuracy.

引用

页码：986 / 989

页数：4

共 16 条

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