Study of a new solid carbon paste tyrosinase-modified amperometric biosensor for the determination of catecholamines by high-performance liquid chromatography

被引：36

作者：

Pravda, M

Petit, C

Michotte, Y

Kauffmann, JM

Vytras, K

机构：

[1] FREE UNIV BRUSSELS, INST PHARMACEUT, B-1050 BRUSSELS, BELGIUM

[2] FREE UNIV BRUSSELS, INST PHARMACEUT, DEPT PHARMACEUT CHEM & DRUG ANAL, B-1090 BRUSSELS, BELGIUM

[3] UNIV PARDUBICE, FAC CHEM TECHNOL, DEPT ANALYT CHEM, CR-53210 PARDUBICE, CZECH REPUBLIC

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 727卷 / 01期

关键词：

biosensors; electrochemical detection; detectors; LC; solid carbon paste electrode; tyrosine-modified electrode; enzyme-immobilized electrodes; catecholamines; dopamine; 3,4-dihydrophenylacetic acid; norepinephrine; homovanillic acid;

D O I：

10.1016/0021-9673(95)01103-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The performance of a tyrosinase-modified ''solid carbon paste'' electrode (SCPE) as electrochemical detector has been studied in comparison with a glassy carbon electrode detector in high-performance liquid chromatography for the simultaneous determination of dopamine, 3,4-dihydroxyphenylacetic acid, norepinephrine and homovanillic acid. The influence of pH, flow-rate and amount of organic solvent in the mobile phase on the biosensor response was investigated. The stability and selectivity of the detector were significantly affected by the mobile phase pH. No effect of 2% isopropanol in the mobile phase was observed. The biosensor response was fast, reproducible, highly sensitive and linear over the concentration range 0.09 mu M(-1) mM (detection limit of ca. 290 pg of neurotransmitter injected).

引用

页码：47 / 54

页数：8

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