The genome of S-PM2, a "photosynthetic" T4-type bacteriophage that infects marine Synechococcus strains

被引:189
作者
Mann, NH [1 ]
Clokie, MRJ
Millard, A
Cook, A
Wilson, WH
Wheatley, PJ
Letarov, A
Krisch, HM
机构
[1] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England
[2] Univ Leicester, Dept Phys & Astron, Leicester LE1 7RH, Leics, England
[3] Plymouth Marine Lab, Plymouth, Devon, England
[4] CNRS, Lab Microbiol & Genet Mol, UMR 5100, Toulouse, France
关键词
D O I
10.1128/JB.187.9.3188-3200.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bacteriophage S-PM2 infects several strains of the abundant and ecologically important marine cyanobacterium Synechococcus. A large lytic phage with an isometric icosahedral head, S-PM2 has a contractile tail and by this criterion is classified as a myovirus (1). The linear, circularly permuted, 196,280-bp double-stranded DNA genome of S-PM2 contains 37.8% G+C residues. It encodes 239 open reading frames (ORFs) and 25 tRNAs. Of these ORFs, 19 appear to encode proteins associated with the cell envelope, including a putative S-layer-associated protein. Twenty additional S-PM2 ORFs have homologues in the genomes of their cyanobacterial hosts. There is a group I self-splicing intron within the gene encoding the D1 protein. A total of 40 ORFs, organized into discrete clusters, encode homologues of T4 proteins involved in virion morphogenesis, nucleotide metabolism, gene regulation, and DNA replication and repair. The S-PM2 genome encodes a few surprisingly large (e.g., 3,779 amino acids) ORFs of unknown function. Our analysis of the S-PM2 genome suggests that many of the unknown S-PM2 functions may be involved in the adaptation of the metabolism of the host cell to the requirements of phage infection. This hypothesis originates from the identification of multiple phage-mediated modifications of the host's photosynthetic apparatus that appear to be essential for maintaining energy production during the lytic cycle.
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页码:3188 / 3200
页数:13
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