Determination of serotonin released from coffee wax by liquid chromatography

被引：16

作者：

Kele, M ^{[1
]}

Ohmacht, R ^{[1
]}

机构：

[1] UNIV PECS,SCH MED,DEPT MED CHEM,H-7643 PECS,HUNGARY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 730卷 / 1-2期

关键词：

coffee; serotonin; pyridine; phenol;

D O I：

10.1016/0021-9673(95)01186-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple hydrolysis and extraction method was developed for the release of serotonin (5-hydroxytryptamine) from a coffee wax sample obtained from decaffeination of coffee beans. The recoverable amount of serotonin was determined by reversed-phase high-performance liquid chromatography with gradient elution and UV detection, using the standard addition method. Different type of basic deactivated chromatographic columns were used for the separation.

引用

页码：59 / 62

页数：4

共 10 条

[1]

BATTINI ML, 1989, ANN CHIM-ROME, V79, P369

[2]

CHENG FC, 1994, ELECTROANAL, V6, P10

[3] RAPID SOLID-PHASE EXTRACTION OF DOPAMINE, SEROTONIN, AND THEIR ACIDIC METABOLITES FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION [J].