High-speed crystal detection and characterization using a fast-readout detector

被引：63

作者：

Aishima, Jun ^{[1
]}

Owen, Robin L. ^{[1
]}

Axford, Danny ^{[1
]}

Shepherd, Emma ^{[1
]}

Winter, Graeme ^{[1
]}

Levik, Karl ^{[1
]}

Gibbons, Paul ^{[1
]}

Ashton, Alun ^{[1
]}

Evans, Gwyndaf ^{[1
]}

机构：

[1] Diamond Light Source, Didcot OX11 0DE, Oxon, England

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2010年 / 66卷

关键词：

macromolecular crystallography; microfocus; grid scan; sample characterization; PROTEIN CRYSTALLOGRAPHY;

D O I：

10.1107/S0907444910028192

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel raster-scanning method combining continuous sample translation with the fast readout of a Pilatus P6M detector has been developed on microfocus beamline I24 at Diamond Light Source. This fast grid-scan tool allows the rapid evaluation of large sample volumes without the need to increase the beam size at the sample through changes in beamline hardware. A slow version is available for slow-readout detectors. Examples of grid-scan use in centring optically invisible samples and in detecting and characterizing numerous microcrystals on a mesh-like holder illustrate the most common applications of the grid scan now in routine use on I24.

引用

页码：1032 / 1035

页数：4

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