Using photoactivatable fluorescent protein Dendra2 to track protein movement

被引：88

作者：

Chudakov, Dmitriy M. ^{[1
]}

Lukyanov, Sergey ^{[1
]}

Lukyanov, Konstantin A. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Bioorgan Chem, Moscow, Russia

来源：

BIOTECHNIQUES | 2007年 / 42卷 / 05期

关键词：

D O I：

10.2144/000112470

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Photoactivatable fluorescent proteins are capable of dramatic changes in fluorescent properties in response to specific light irradiation. For example, they can be converted from cyan to green, or from green to red, or from nonfluorescent to a brightly fluorescent state. Several types of such proteins were developed recently, and some of them are already becoming popular tools to study protein mobility. Here we provide detailed recommendations on application of the monomeric green-to-red photoconvertible fluorescent protein Dendra2 for protein tracking in living cultured cells.

引用

页码：553 / +

页数：7

共 30 条

[11] Fast and reversible photoswitching of the fluorescent protein Dronpa as evidenced by fluorescence correlation spectroscopy [J].

Dedecker, Peter ;

Hotta, Jun-ichi ;

Ando, Ryoko ;

Miyawaki, Atsushi ;

Engelborghs, Yves ;

Hofkens, Johan .

BIOPHYSICAL JOURNAL, 2006, 91 (05) :L45-L47

[12] Monitoring dynamic protein interactions with photoquenching FRET [J].

Demarco, Ignacio A. ;

Periasamy, Ammasi ;

Booker, Cynthia F. ;

Day, Richard N. .

NATURE METHODS, 2006, 3 (07) :519-524

[13] Ground-state structures and vertical excitations for the kindling fluorescent protein asFP595 [J].

Grigorenko, Bella ;

Savitsky, Alexander ;

Topol, Igor ;

Burt, Stanley ;

Nemukhin, Alexander .

JOURNAL OF PHYSICAL CHEMISTRY B, 2006, 110 (37) :18635-18640

[14] Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light [J].

Gurskaya, NG ;

Verkhusha, VV ;

Shcheglov, AS ;

Staroverov, DB ;

Chepurnykh, TV ;

Fradkov, AF ;

Lukyanov, S ;

Lukyanov, KA .

NATURE BIOTECHNOLOGY, 2006, 24 (04) :461-465

[15] Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: mechanism responsible for the reversible photoswitching [J].

Habuchi, Satoshi ;

Dedecker, Peter ;

Hotta, Jun-ichi ;

Flors, Cristina ;

Ando, Ryoko ;

Mizuno, Hideaki ;

Miyawaki, Atsushi ;

Hofkens, Johan .

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2006, 5 (06) :567-576

[16] Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins [J].

Hofmann, M ;

Eggeling, C ;

Jakobs, S ;

Hell, SW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (49) :17565-17569

[17]

Lippincott-Schwartz J, 2003, NAT CELL BIOL, pS7, DOI 10.1038/ncb1032

[18] Natural animal coloration can be determined by a nonfluorescent green fluorescent protein homolog [J].

Lukyanov, KA ;

Fradkov, AF ;

Gurskaya, NG ;

Matz, MV ;

Labas, YA ;

Savitsky, AP ;

Markelov, ML ;

Zaraisky, AG ;

Zhao, XN ;

Fang, Y ;

Tan, WY ;

Lukyanov, SA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (34) :25879-25882

[19] Photoactivatable fluorescent proteins [J].

Lukyanov, KA ;

Chudakov, DM ;

Lukyanov, S ;

Verkhusha, VV .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2005, 6 (11) :885-891

[20] A photoactivatable GFP for selective photolabeling of proteins and cells [J].

Patterson, GH ;

Lippincott-Schwartz, J .

SCIENCE, 2002, 297 (5588) :1873-1877

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