Probing of DNA and single-base mismatches by chemical force microscopy using peptide nucleic acid-modified sensing tips and functionalized surfaces

被引：16

作者：

Lioubashevski, O ^{[1
]}

Patolsky, F ^{[1
]}

Willner, I ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

来源：

LANGMUIR | 2001年 / 17卷 / 17期

关键词：

D O I：

10.1021/la0105174

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

DNA hybridization to peptide nucleic acid (PNA)-functionalized Au-coated tips is characterized by probing the force interactions between the PNA-functionalized tip or the DNA/PNA-functionalized tip with a hydrophobic 1-mercaptoundecane-modified Au surface, using chemical force microscopy (CFM). DNA concentrations as low as 3 x 10(-13) M can be detected by probing the respective force interactions. The CFM method is sufficiently sensitive to detect a single base mismatch,in the analyte DNA.

引用

页码：5134 / 5136

页数：3

共 29 条

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