Release of IL-1β via IL-1β-converting enzyme in a skin dendritic cell line exposed to 2,4-dinitrofluorobenzene

被引：9

作者：

Matos, TJ

Jaleco, SP

Gonçalo, M

Duarte, CB

Lopes, MC

机构：

[1] Univ Coimbra, Fac Farm, Hosp Univ Coimbra, P-3000295 Coimbra, Portugal

[2] Univ Coimbra, Ctr Neurosci & Biol Celular, P-3004517 Coimbra, Portugal

[3] Univ Coimbra, Dept Zool, P-3004517 Coimbra, Portugal

[4] Univ Coimbra, Fac Farm, P-3000295 Coimbra, Portugal

来源：

MEDIATORS OF INFLAMMATION | 2005年 / 03期

关键词：

D O I：

10.1155/MI.2005.131

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We used a mouse fetal skin dendritic cell line (FSDC) to study the effect of the strong allergen 2,4-dinitrofluorobenzene (DNFB) on interleukin (IL)-1 beta release and IL-1 beta receptor immuno reactivity. Stimulation with DNFB (30 minutes) increased IL-1 beta release without changing the mRNA levels of the protein. Furthermore, DNFB increased transiently the interleukin-1 beta-converting enzyme (ICE) activity, as measured with its fluorogenic substrate Z-Tyr-Val-Ala-Asp-AFC. The ICE inhibitor Z-YVAD-FMK prevented the release of IL-1 beta evoked by DNFB. Incubation of the cells with DNFB (30 minutes) strongly increased IL-1 beta receptor immunoreac- tivity. The rapid effect of DNFB on the release of mature ILAP, without inducing an increase of IL-1 beta mRNA in FSDC, suggests a posuranslational modification of pro-IL-1 beta by ICE activity.

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页码：131 / 138

页数：8

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