Application of high-performance anion-exchange chromatography with pulsed amperometric detection and statistical analysis to study oligosaccharide distributions -: a complementary method to investigate the structure and some properties of alginates

被引：33

作者：

Ballance, S

Holtan, S

Aarstad, OA

Sikorski, P

Skjåk-Bræk, G

Christensen, BE

机构：

[1] Norwegian Univ Sci & Technol, NTNU, Norwegian Biopolymer Lab, NOBIPOL,Dept Biotechnol, N-7491 Trondheim, Norway

[2] Norwegian Univ Sci & Technol, NTNU, Dept Phys, NOBIPOL, N-7491 Trondheim, Norway

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1093卷 / 1-2期

关键词：

acid hydrolysis; carbohydrate; chain-length distribution; degree of polymerisation;

D O I：

10.1016/j.chroma.2005.07.051

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Alginates comprised of essentially alternating units of mannuronic (M) acid-guluronic (G) acid (MG-alginate), and G-blocks isolated from a seaweed where subjected to partial acid hydrolysis at pH 3.5 The chain-length distribution of oligosaccharides in the hydrolysate were investigated by statistical analysis after their separation with high-performance anion-exchange chromatography and pulsed amperometric detection (HPAEC-PAD). Simulated depolymerisation of the MG-alginate provided an estimate of the ratio between two acid hydrolysis rate constants (p = 8.3 +/- 1) and the average distribution of the MM linkages in the original sample of polysaccharide chains. In conclusion, we found HPAEC-PAD together with statistical analysis was a useful method to investigate the fine structure and some properties of binary polysaccharides. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：59 / 68

页数：10

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