Microbial hydrogen production from sweet potato starch residue

被引:262
作者
Yokoi, H [1 ]
Saitsu, A [1 ]
Uchida, H [1 ]
Hirose, J [1 ]
Hayashi, S [1 ]
Takasaki, Y [1 ]
机构
[1] Miyazaki Univ, Fac Engn, Dept Appl Chem, Miyazaki 8892192, Japan
关键词
hydrogen; hydrogen production; sweet potato starch; starch residue;
D O I
10.1263/jbb.91.58
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Clostridium Butyricum could produce hydrogen from a sweet potato starch residue upon supplementation of nitrogen sources. A repeated batch culture using a mixed culture of C. butyricum and Enterobacter aerogenes produced hydrogen with a yield of 2.4 mol H-2/mol glucose under a controlled culture pH of 5.25 in a medium consisting of the sweet potato starch residue and 0.1% Polypepton without addition of any reducing agents. Rhodobacter sp. M-19 produced hydrogen from the supernatant of the culture broth obtained in the repeated batch culture containing C. butyricum and E. aerogenes when 50 mug/l Na2MoO4. 2H(2)O and 20 mg/l EDTA were added to the supernatant and it was cultured under a controlled culture pH of 7.5. A high yield of hydrogen of 7.0 mol H-2/mol glucose from the starch remaining in the starch residue was attained in two-step repeated batch cultures containing C. butyricum and E. aerogenes, and by Rhodobacter sp. M-19.
引用
收藏
页码:58 / 63
页数:6
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