Highly active and stable DNAzyme - Carbon nanotube hybrids

被引：85

作者：

Yim, TJ

Liu, JW

Lu, Y

Kane, RS ^{[1
]}

Dordick, JS

机构：

[1] Rensselaer Polytech Inst, Dept Chem & Biol Engn, Urbana, IL 61801 USA

[2] Rensselaer Polytech Inst, Dept Biol, Urbana, IL 61801 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 35期

关键词：

D O I：

10.1021/ja0541581

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report herein the generation and characterization of DNAzyme-carbon nanotube conjugates. Biotinylated DNAzyme was attached to streptavidin-functionalized MWNTs. The resultant DNAzyme-MWNT conjugates exhibited Michaelis-Menten kinetics and multiple catalytic turnovers. The specific activity of the nanoscale conjugates was ∼1/3 of that for freely soluble DNAzyme. Such high activity may allow myriad applications ranging from the directed assembly of nanomaterials to nanoscale cellular therapeutics that rely on the RNA cleaving ability of DNAzymes. Copyright © 2005 American Chemical Society.

引用

页码：12200 / 12201

页数：2

共 20 条

[1]

Balavoine F, 1999, ANGEW CHEM INT EDIT, V38, P1912, DOI 10.1002/(SICI)1521-3773(19990712)38:13/14<1912::AID-ANIE1912>3.0.CO

[2]

2-2

[3]

Breaker R R, 1994, Chem Biol, V1, P223, DOI 10.1016/1074-5521(94)90014-0

[4] Characterization of grafting density and binding efficiency of DNA and proteins on gold surfaces [J].