Inhibitory effects of indomethacin on interleukin-1 and nitric oxide production in rat microglia in vitro

被引：32

作者：

Du, ZY ^{[1
]}

Li, XY ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China

来源：

INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY | 1999年 / 21卷 / 03期

关键词：

indomethacin; interleukin-1; tumor necrosis factor-alpha; nitric oxide; microglia;

D O I：

10.1016/S0192-0561(98)00084-8

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Indomethacin, as a nonsteroidal antiinflammatory drug, is reported to be effective in some degree in the prevention and treatment of Alzheimer's disease (AD). Effects of indomethacin on proinflammatory cytokines interleukin-1 (IL-1), tumor necrosis factor alpha and nitric oxide (NO) on rat microglia in vitro were investigated. The biological activities of IL-1 were assayed by thymocyte proliferation assay and the activities of TNG-alpha, by L929 cytotoxicity assay. NO concentration was represented by nitrite and determined by Griess reaction. Indomethacin inhibited IL-1 and NO production by rat microglia stimulated at the concentration of 0.1-10 mu mol/l. However, it did not show any inhibitory effect on TNF-alpha production by resting and LPS-stimulated rat microglia. The results suggest that the mechanism by which indomethacin might be beneficial in treatment of AD might be due to the inhibition IL-1 and NO production from microglia. (C) 1999 International Society for Immunopharmacology. Published by Elsevier Science Ltd.

引用

页码：219 / 225

页数：7

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