Legume symbiotic nitrogen fixation by β-proteobacteria is widespread in nature

被引:276
作者
Chen, WM
Moulin, L
Bontemps, C
Vandamme, P
Béna, G
Boivin-Masson, C
机构
[1] INRA, CNRS, Lab Interact Plantes Microorganismes, F-31326 Castanet Tolosan, France
[2] Natl Kaohsiung Inst Marine Technol, Dept Seafood Sci, Microbiol Lab, Kaohsiung 811, Taiwan
[3] ENSAM, CIRAD, INRA, IRD,LSTM, F-34398 Montpellier, France
[4] State Univ Ghent, Microbiol Lab, B-9000 Ghent, Belgium
关键词
D O I
10.1128/JB.185.24.7266-7272.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Following the initial discovery of two legume-nodulating Burkholderia strains (L. Moulin, A. Munive, B. Dreyfus, and C. Boivin-Masson, Nature 411:948-950, 2001), we identified as nitrogen-fixing legume symbionts at least 50 different strains of Burkholderia caribensis and Ralstonia taiwanensis, all belonging to the beta-subclass of proteobacteria, thus extending the phylogenetic diversity of the rhizobia. R. taiwanensis was found to represent 93% of the Mimosa isolates in Taiwan, indicating that beta-proteobacteria can be the specific symbionts of a legume. The nod genes of rhizobial beta-proteobacteria (beta-rhizobia) are very similar to those of rhizobia from the alpha-subclass (alpha-rhizobia), strongly supporting the hypothesis of the unique origin of common nod genes. The beta-rhizobial nod genes are located on a 0.5-Mb plasmid, together with the nifH gene, in R. taiwanensis and Burkholderia phymatum. Phylogenetic analysis of available nodA gene sequences clustered beta-rhizobial sequences in two nodA lineages intertwined with alpha-rhizobial sequences. On the other hand, the beta-rhizobia were grouped with free-living nitrogen-fixing beta-proteobacteria on the basis of the nifH phylogenetic tree. These findings suggest that beta-rhizobia evolved from diazotrophs through multiple lateral nod gene transfers.
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页码:7266 / 7272
页数:7
相关论文
共 27 条
[21]   Methylotrophic Methylobacterium bacteria nodulate and fix nitrogen in symbiosis with legumes [J].
Sy, A ;
Giraud, E ;
Jourand, P ;
Garcia, N ;
Willems, A ;
de Lajudie, P ;
Prin, Y ;
Neyra, M ;
Gillis, M ;
Boivin-Masson, C ;
Dreyfus, B .
JOURNAL OF BACTERIOLOGY, 2001, 183 (01) :214-220
[22]   The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools [J].
Thompson, JD ;
Gibson, TJ ;
Plewniak, F ;
Jeanmougin, F ;
Higgins, DG .
NUCLEIC ACIDS RESEARCH, 1997, 25 (24) :4876-4882
[23]  
Tripathi AK, 2002, CURR SCI INDIA, V82, P8
[24]   THE RHIZOBIUM-LEGUME SYMBIOSIS - 2 METHODS TO DISCRIMINATE BETWEEN NODULES AND OTHER ROOT-DERIVED STRUCTURES [J].
TRUCHET, G ;
CAMUT, S ;
DEBILLY, F ;
ODORICO, R ;
VASSE, J .
PROTOPLASMA, 1989, 149 (2-3) :82-88
[25]   The glutamine synthetases of rhizobia: Phylogenetics and evolutionary implications [J].
Turner, SL ;
Young, JPW .
MOLECULAR BIOLOGY AND EVOLUTION, 2000, 17 (02) :309-319
[26]   Burkholderia tuberum sp nov and Burkholderia phymatum sp nov., nodulate the roots of tropical legumes [J].
Vandamme, P ;
Goris, J ;
Chen, WM ;
de Vos, P ;
Willems, A .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2002, 25 (04) :507-512
[27]   The common nodulation genes of Astragalus sinicus rhizobia are conserved despite chromosomal diversity [J].
Zhang, XX ;
Turner, SL ;
Guo, XW ;
Yang, HJ ;
Debelló, F ;
Yang, GP ;
Dénarié, J ;
Young, JPW ;
Li, FD .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (07) :2988-2995