Extraction and analysis of ellagic acid from novel complex sources

被引:48
作者
Aguilera-Carbo, Antonio F. [2 ]
Augur, Christopher [3 ]
Prado-Barragan, Lilia A. [2 ]
Aguilar, Cristobal N. [1 ]
Favela-Torres, Ernesto [2 ]
机构
[1] Univ Autonoma Coahuila, Sch Chem, Food Res Dept, Saltillo 25280, Coahuila, Mexico
[2] Univ Autonoma Metropolitana Iztapalapa, Dept Biotechnol, Iztapalapa 09340, DF, Mexico
[3] Univ Paul Cezanne, Fac Sci St Jerome, IMEP Case 441, IRD Unite Biotrans, F-13397 Marseille 20, France
来源
CHEMICAL PAPERS | 2008年 / 62卷 / 04期
关键词
ellagic acid; semiarid plants; HPLC analysis;
D O I
10.2478/s11696-008-0042-y
中图分类号
O6 [化学];
学科分类号
0703 [化学];
摘要
Ellagic acid (EA) was quantified by reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with photodiode array detection (DAD) in five fine-powdered plants collected from the semiarid region of Mexico. Samples analysed included Jatropha dioica branches (Dragon's blood), Euphorbia antisyphyllitica branches (Candelilla), Turnera diffusa Willd leaves (Damiana), Flourensia cernua leaves (hojasen) and Punica granatum husk (pomegranate) at two maturity stages ("turning" or intermediate and maturated fruit, considered as positive controls). The results demonstrated high EA concentrations in all tested samples which are novel sources of this natural antioxidant. The method developed for the EA analysis is fast and it showed an excellent linearity range, repeatability, intra-and inter-day precision and accuracy with respect to the methods reported for the EA analysis.
引用
收藏
页码:440 / 444
页数:5
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