Expression of vesicular glutamate transporters in rat lumbar spinal cord, with a note on dorsal root ganglia

被引:116
作者
Landry, M
Bouali-Benazzouz, R
El Mestikawy, S
Ravassard, P
Nagy, F
机构
[1] Univ Bordeaux 2, INSERM, Inst Francois Magendie, F-33077 Bordeaux, France
[2] Hop La Pitie Salpetriere, Inst Natl Sante Rech Med, U 106, F-35651 Paris, France
[3] Hop La Pitie Salpetriere, Ctr Natl Rech Sci, UMR 7091, F-75013 Paris, France
关键词
colocalization; excitatory interneuron; motoneuron; sensory neuron;
D O I
10.1002/cne.10988
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Three vesicular glutamate transporters (VGLUTs) have been recently identified and their distribution has been mapped in various brain areas. In the present study, we used morphological approaches to investigate their expression in the rat lumbar spinal cord and dorsal root ganglia. Our results show a complementary distribution of VGLUT-expressing fibers in the spinal cord, with no overlapping in nerve endings. In the dorsal horn, VGLUT1 is most abundant in mechanosensory/proprioceptive deep afferent fibers. VGLUT2 and VGLUT3 are expressed only at moderate levels in primary sensory afferent fibers and are not used by central projections of nociceptive neurons. VGLUT1 and VGLUT2 mRNAs are mainly segregated in superficial laminae but colocalized in deeper laminae. Weak expression of VGLUT3 mRNA is only detected in deep laminae. The colocalization of VGLUT1 and VGLUT2 transcripts in most sensory neurons of the dorsal root ganglia is not in agreement with the clear segregation between the proteins in their spinal projections. Such a discrepancy suggests targeting mechanisms specific for each transporter and/or a distinct regulation of their translation. In the ventral horn, the expression of VGLUT1 and VGLUT2 mRNAs in motoneuron perikarya suggests the possible unexpected role of glutamate in the vertebrate neuromuscular junction. These results demonstrate the existence of different subpopulations of glutamate nerve terminals in the rat lumbar spinal cord and suggest that functionally distinct subsets of excitatory glutamatergic neuronal networks are involved in sensory processing and motor control. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:380 / 394
页数:15
相关论文
共 41 条
[31]   Identification of differentiation-associated brain-specific phosphate transporter as a second vesicular glutamate transporter (VGLUT2) [J].
Takamori, S ;
Rhee, JS ;
Rosenmund, C ;
Jahn, R .
JOURNAL OF NEUROSCIENCE, 2001, 21 (22)
[32]   Molecular cloning and functional characterization of human vesicular glutamate transporter 3 [J].
Takamori, S ;
Malherbe, P ;
Broger, C ;
Jahn, R .
EMBO REPORTS, 2002, 3 (08) :798-803
[33]   The expression of vesicular glutamate transporters VGLUT1 and VGLUT2 in neurochemically defined axonal populations in the rat spinal cord with emphasis on the dorsal horn [J].
Todd, AJ ;
Hughes, DI ;
Polgár, E ;
Nagy, GG ;
Mackie, M ;
Ottersen, OP ;
Maxwell, DJ .
EUROPEAN JOURNAL OF NEUROSCIENCE, 2003, 17 (01) :13-27
[34]   Motor coordination without action potentials in the mammalian spinal cord [J].
Tresch, MC ;
Kiehn, O .
NATURE NEUROSCIENCE, 2000, 3 (06) :593-599
[35]   MODULATION OF SPINAL EXCITABILITY - COOPERATION BETWEEN NEUROKININ AND EXCITATORY AMINO-ACID NEUROTRANSMITTERS [J].
URBAN, L ;
THOMPSON, SWN ;
DRAY, A .
TRENDS IN NEUROSCIENCES, 1994, 17 (10) :432-438
[36]   Identification of the differentiation-associated Na+/PI transporter as a novel vesicular glutamate transporter expressed in a distinct set of glutamatergic synapses [J].
Varoqui, H ;
Schäfer, MKH ;
Zhu, HM ;
Weihe, E ;
Erickson, JD .
JOURNAL OF NEUROSCIENCE, 2002, 22 (01) :142-155
[37]  
WAERHAUG O, 1993, ANAT EMBRYOL, V188, P501
[38]  
Willis W. D., 1991, SENSORY MECH SPINAL
[39]   Chemical activators of sensory neurons [J].
Wood, JN ;
Docherty, R .
ANNUAL REVIEW OF PHYSIOLOGY, 1997, 59 :457-482
[40]  
Woolf C J, 1994, Drugs, V47 Suppl 5, P1, DOI 10.2165/00003495-199400475-00003